Escherichia coli O157:H7 Genetic Diversity in Bovine Fecal Samples

Escherichia coli O157:H7 causes foodborne illness in humans; cattle are considered a primary reservoir for the organism, and transmission is often through contaminated food products or water. The objective of this study was to determine the genetic diversity of E. coli O157:H7 within a single indivi...

Full description

Saved in:
Bibliographic Details
Published in:Journal of food protection 2011-07, Vol.74 (7), p.1186-1188
Main Authors: JACOB, M. E, ALMES, K. M, SHI, X, SARGEANT, J. M, NAGARAJA, T. G
Format: Article
Language:English
Subjects:
Animals
Beef
Beef cattle
Biological and medical sciences
Carcasses
Carrier State - microbiology
Carrier State - veterinary
Cattle
Cattle - microbiology
Colonies
Consumer Product Safety
Dairies
Disease Reservoirs - microbiology
Disease Reservoirs - veterinary
E coli
Electrophoresis
Electrophoresis, Gel, Pulsed-Field
Escherichia coli
Escherichia coli Infections - prevention & control
Escherichia coli O157 - classification
Escherichia coli O157 - genetics
Escherichia coli O157 - pathogenicity
Fecal coliforms
Feces
Feces - microbiology
Food
Food contamination
Food contamination & poisoning
Food Contamination - prevention & control
Food industries
Food Microbiology
Food products
Food safety
food-borne diseases
Foodborne diseases
Fundamental and applied biological sciences. Psychology
Genes
Genetic diversity
Genetic Variation
Humans
immunomagnetic separation
Phylogeny
Polymerase chain reaction
Prevalence
Pulsed-field gel electrophoresis
Reservoirs
Studies
Typing
Virulence
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Escherichia coli O157:H7 causes foodborne illness in humans; cattle are considered a primary reservoir for the organism, and transmission is often through contaminated food products or water. The objective of this study was to determine the genetic diversity of E. coli O157:H7 within a single individual bovine fecal sample based on pulsed-field gel electrophoresis (PFGE) typing. Fecal samples (n=601) were collected from dairy and beef cattle at three separate facilities, and E. coli O157:H7 was isolated by enrichment, immunomagnetic separation, and plating on selective medium. The prevalence of E. coli O157:H7 was 46 (7.7%) of 601. From each positive fecal sample, up to 10 putative colonies were tested, and isolates from samples with at least seven positive colonies were subtyped using PFGE and tested for six major virulence genes by multiplex PCR. A total of 254 E. coli O157:H7 isolates from 27 samples met these criteria and were included in PFGE analysis. Fifteen PFGE subtypes (
ISSN:0362-028X
1944-9097
DOI:10.4315/0362-028X.JFP-11-022