Costimulatory strength influences the differential effects of transforming growth factor beta 1 for the generation of CD8 super(+ regulatory T cells)

• Published in: Molecular immunology 2008-05, Vol.45 (10), p.2937-2950
• Main Authors: Fan, Timothy M, Kranz, David M, Flavell, Richard A, Roy, Edward J
• Format: Article
• Language: English
• ISSN: 0161-5890
• DOI: 10.1016/j.molimm.2008.01.019

Transforming growth factor beta 1 (TGF beta 1) is a pleiotropic cytokine, capable of exerting diverse biologic effects. Despite its central role in multiple immune activities, the molecular signals responsible for shaping TGF beta 1's immunologic properties remain poorly elucidated. We report that costimulatory strength acts as a molecular switch, which influences the differential effects of TGF beta 1 on the effector and regulatory development of naieve CD8 super(+ lymphocytes. At low costimulation, TGF beta 1 inhibits proliferation of CD8) super(+) lymphocytes and cytokine secretion, but at high costimulation the response to TGF beta 1 is quite different. High costimulation combined with TGF beta 1 generates CD8 super(+CD25) super(+) T lymphocytes which maintain robust proliferative and survival capacity in the presence of low IL-2 concentrations. Furthermore, under these conditions, a subpopulation of CD8 super(+ T lymphocytes is generated that expressFoxp3, secrete IL-10, and inhibit naieve T lymphocyte proliferation via a contact-dependent mechanism. The adoptive transfer of these CD8) super(+)CD25 super(+Foxp3) super(+) T cells into mice inoculated intravenously with B16F10 melanoma appears to accelerate tumor progression as reflected by an increase in the number of pulmonary metastatic tumor foci. These findings indicate that costimulatory strength may act as a molecular switch in the generation of CD8 super(+ T cells which possess a regulatory phenotype and the capacity to reduce antitumor immune responses within tumor-bearing mice.)