Variation in male reproductive traits among three bitterling fishes (Acheilognathinae: Cyprinidae) in relation to the mating system

• Published in: Biological journal of the Linnean Society 2011-07, Vol.103 (3), p.622-632
• Main Authors: PATEMAN-JONES, CHRISTOPHER, RASOTTO, MARIA BERICA, REICHARD, MARTIN, LIAO, CAIPING, LIU, HUANZHANG, ZIĘBA, GRZEGORZ, SMITH, CARL
• Format: Article
• Language: English
• Subjects: Acheilognathinae; Agnatha. Pisces; Biological and medical sciences; Biological evolution; Cyprinidae; ejaculation; fish; Freshwater; Fundamental and applied biological sciences. Psychology; Genetics of eukaryotes. Biological and molecular evolution; mating systems; reproduction; reproductive traits; Rhodeus amarus; Rhodeus ocellatus; Rhodeus sinensis; risk; Rosa chinensis; species differences; sperm competition; sperm depletion; sperm economy; sperm limitation; sperm motility; spermatozoa; tail; temporal variation; testes; Vertebrates: general zoology, morphology, phylogeny, systematics, cytogenetics, geographical distribution
• ISSN: 0024-4066; 1095-8312
• DOI: 10.1111/j.1095-8312.2011.01648.x

Male traits that correlate with fertilization success include testis size and structure, ejaculate size, ejaculation frequency, and sperm motility. Two hypotheses potentially explain interspecific differences in these traits: sperm competition and sperm limitation. We examined variation in six traits associated with fertilization success in three closely-related species of bitterling fish; the European bitterling (Rhodeus amarus), the Chinese rose bitterling (Rhodeus ocellatus), and the Chinese bitterling (Rhodeus sinensis). Interspecific differences indicated that the three study species have evolved different sperm allocation strategies. Rhodeus amarus displayed the most developed reproductive apparatus with a number of traits associated with both high levels of sperm production and fertilization efficiency. Rhodeus ocellatus and R. sinensis appear to have more comparable sperm allocation strategies, although relative testis size and spermatozoa head : tail ratio were greater in R. sinensis, suggesting that sperm competition risk may be higher in this species. All three species possessed an unusually well developed sperm duct with evidence of mucin production, which greatly extends the longevity of sperm and, consequently, the period over which fertilization can occur. We discuss these findings in the context of differences in the mating systems of the species examined, and relate the results obtained to differences in the temporal and spatial clustering of fertilizations. © 2011 The Linnean Society of London, Biological Journal of the Linnean Society, 2011, 103, 622-632.