Loading…
Polymorphic minisatellites in the mitochondrial DNAs of Oryza and Brassica
Polymorphic analyses of angiosperm mitochondrial DNA are rare in comparison with chloroplast DNA, because few target sequences in angiosperm mitochondrial DNA are known. Minisatellites, a tandem array of repeated sequences with a repeat unit of 10 to ~100 bp, are popular target sequences of animal m...
Saved in:
Published in: | Current genetics 2011-08, Vol.57 (4), p.261-270 |
---|---|
Main Authors: | , , , , , |
Format: | Article |
Language: | English |
Subjects: | |
Citations: | Items that this one cites Items that cite this one |
Online Access: | Get full text |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
Summary: | Polymorphic analyses of angiosperm mitochondrial DNA are rare in comparison with chloroplast DNA, because few target sequences in angiosperm mitochondrial DNA are known. Minisatellites, a tandem array of repeated sequences with a repeat unit of 10 to ~100 bp, are popular target sequences of animal mitochondria, but
Beta vulgaris
is the only known angiosperm species for which such an analysis has been conducted. From this lack of information, it was uncertain as to whether polymorphic minisatellites existed in other angiosperm species. Ten plant mitochondrial DNAs were found to contain minisatellite-like repeated sequences, most of which were located in intergenic regions but a few occurred in gene coding and intronic regions.
Oryza
and
Brassica
accessions were selected as models for the investigation of minisatellite polymorphism because substantial systematic information existed. PCR analysis of 42
Oryza
accessions revealed length polymorphisms in four of the five minisatellites. The mitochondrial haplotypes of the 16
Oryza
accessions with chromosomal complement (genome) types of CC, BBCC and CCDD were identical but were clearly distinguished from BB-genome accessions, a result consistent with the notion that the cytoplasmic donor parent of the amphidiploid species might be the CC-genome species. Twenty-nine accessions of six major cultivated species of
Brassica
were classified into five mitochondrial haplotypes based on two polymorphic minisatellites out of six loci. The haplotypes of
Brassica juncea
and
Brassica carinata
accessions were identical to
Brassica rapa
and
Brassica nigra
accessions, respectively. The haplotypes of
Brassica napus
accessions were heterogeneous and unique, results that were consistent with previous studies. |
---|---|
ISSN: | 0172-8083 1432-0983 |
DOI: | 10.1007/s00294-011-0345-3 |