Proteomic study of Carissa spinarum in response to combined heat and drought stress

Carissa spinarum is one of the secondary advantage plants grown in dry-hot valleys in China, which can survive under stress conditions of high temperature and extreme low humidity. Here, we studied the physiological and proteomic changes of C. spinarum in response to 42°C heat stress treatment in co...

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Bibliographic Details
Published in:Proteomics (Weinheim) 2010-09, Vol.10 (17), p.3117-3129
Main Authors: Zhang, Minhua, Li, Gwowei, Huang, Wei, Bi, Ting, Chen, Genyun, Tang, Zhangcheng, Su, Weiai, Sun, Weining
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Analytical, structural and metabolic biochemistry
Apocynaceae - metabolism
Apocynaceae - physiology
Biological and medical sciences
Carissa
Carissa spinarum
Chaperonins - chemistry
Chaperonins - metabolism
Cluster Analysis
Droughts
Electrophoresis, Gel, Two-Dimensional
Fundamental and applied biological sciences. Psychology
Heat and drought
Heat-Shock Proteins - chemistry
Heat-Shock Proteins - metabolism
Heat-Shock Response - physiology
Hot Temperature
Mass Spectrometry
Miscellaneous
Molecular Sequence Data
Plant Leaves - chemistry
Plant Leaves - metabolism
Plant Proteins - chemistry
Plant Proteins - classification
Plant Proteins - metabolism
Plant proteomics
Proteins
Proteome - chemistry
Proteomics - methods
Reproducibility of Results
Small HSP
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Summary:Carissa spinarum is one of the secondary advantage plants grown in dry-hot valleys in China, which can survive under stress conditions of high temperature and extreme low humidity. Here, we studied the physiological and proteomic changes of C. spinarum in response to 42°C heat stress treatment in combination with drought stress. Dynamic changes in the leaf proteome were analyzed at four time points during the stress treatment and recovery stages. Approximately, 650 protein spots were reproducibly detected in each gel. Forty-nine spots changed their expression levels upon heat and drought treatment, and 30 proteins were identified by MS and 2-D Western blot. These proteins were classified into several categories including HSP, photosynthesis-related protein, RNA-processing protein and proteins involved in metabolism and energy production. The potential roles of these stress-responsive proteins are discussed.
ISSN:1615-9853
1615-9861
1615-9861
DOI:10.1002/pmic.200900637