Deletion of the virion host shut-off gene of pseudorabies virus results in selective upregulation of the expression of early viral genes in the late stage of infection

A real-time RT-PCR technique was applied to evaluate the impact of deletion of the virion host shut-off (VHS) gene on the kinetics of pseudorabies virus gene expression. Selective suppression of early gene transcripts by the viral ribonuclease occurs after 4 h of infection; while VHS protein appears...

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Bibliographic Details
Published in:Genomics (San Diego, Calif.) Calif.), 2011-07, Vol.98 (1), p.15-25
Main Authors: TOMBACZ, Dóra, TOTH, Judit S, BOLDOGKÖI, Zsolt
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Cell Line
Diverse techniques
Fundamental and applied biological sciences. Psychology
Gene Deletion
gene expression
gene expression regulation
Gene Expression Regulation, Viral
genes
Genes. Genome
Genetics of eukaryotes. Biological and molecular evolution
Herpesvirus
Herpesvirus 1, Suid - genetics
Kinetics
messenger RNA
Molecular and cellular biology
Molecular genetics
Pseudorabies virus
Real-time PCR
reverse transcriptase polymerase chain reaction
ribonucleases
Ribonucleases - genetics
RNA, Messenger - genetics
Suid herpesvirus 1
Swine
Transcription, Genetic
Up-Regulation
Viral Proteins - genetics
virion
Virion host shut-off
viruses
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Summary:A real-time RT-PCR technique was applied to evaluate the impact of deletion of the virion host shut-off (VHS) gene on the kinetics of pseudorabies virus gene expression. Selective suppression of early gene transcripts by the viral ribonuclease occurs after 4 h of infection; while VHS protein appears to act non-selectively on the transcripts belonging in different kinetic classes in the first 2 h of infection. VHS protein disrupts the close correlation between the transcription kinetics of the immediate-early 180 protein and the other pseudorabies virus transcripts. The typical pattern of early gene expression was found to be altered in the VHS gene-deleted virus in that the production rates of their transcripts did not decline from 4 h post-infection. This observation led us to put forward the hypothesis that the VHS protein may play a pivotal role in the switch from the early to the late stage of infection.
ISSN:0888-7543
1089-8646
DOI:10.1016/j.ygeno.2011.03.010