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Molecular characterization of interleukin-6 in the gilthead seabream ( Sparus aurata)
A cDNA clone, designated sbIL-6 (seabream interleukin-6), was obtained from a cDNA library of enriched immune-stimulated sequences from gilthead seabream. The deduced sbIL-6 protein corresponds to a 225-amino acid protein with a putative 24-amino acid signal peptide, four conserved alpha helices and...
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Published in: | Molecular immunology 2008-07, Vol.45 (12), p.3363-3370 |
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container_title | Molecular immunology |
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creator | Castellana, Barbara Iliev, Dimitar B. Sepulcre, M. Pilar MacKenzie, Simon Goetz, Frederick W. Mulero, Victoriano Planas, Josep V. |
description | A cDNA clone, designated sbIL-6 (seabream interleukin-6), was obtained from a cDNA library of enriched immune-stimulated sequences from gilthead seabream. The deduced sbIL-6 protein corresponds to a 225-amino acid protein with a putative 24-amino acid signal peptide, four conserved alpha helices and one
N-linked glycosylation site. At the amino acid level sbIL-6 shares 23–26% identity with mammalian IL-6 sequences and 30–51% identity with other fish IL-6 sequences. The structure of the sbIL-6 gene consisted of 5 exons and 4 introns, spanning 2.4
kb. Healthy fish expressed sbIL-6 in white muscle, skin, spleen, anterior intestine and stomach, while no expression was detected in brain, gill, head kidney, posterior intestine and adipose tissue. A significant up-regulation of sbIL-6 expression was observed after lipopolysaccharide (LPS),
Vibrio anguillarum DNA (
VaDNA) and peptidoglycan treatment in cultured seabream head kidney leukocytes. Using purified immune cells, sbIL-6 expression was induced similarly in macrophages and acidophilic granulocytes by
VaDNA but LPS was more effective in inducing sbIL-6 expression in acidophilic granulocytes than in macrophages. Furthermore,
in vivo infection of seabream with live
V. anguillarum caused significant increases in sbIL-6 mRNA expression in the thymus, peritoneal exudate, head kidney and gills. In summary, our study provides further evidence for the existence of distinct IL-6 genes in lower vertebrates and for the strong induction of their expression by immune stimuli, supporting the notion of a potentially important role for this cytokine in fish. |
doi_str_mv | 10.1016/j.molimm.2008.04.012 |
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N-linked glycosylation site. At the amino acid level sbIL-6 shares 23–26% identity with mammalian IL-6 sequences and 30–51% identity with other fish IL-6 sequences. The structure of the sbIL-6 gene consisted of 5 exons and 4 introns, spanning 2.4
kb. Healthy fish expressed sbIL-6 in white muscle, skin, spleen, anterior intestine and stomach, while no expression was detected in brain, gill, head kidney, posterior intestine and adipose tissue. A significant up-regulation of sbIL-6 expression was observed after lipopolysaccharide (LPS),
Vibrio anguillarum DNA (
VaDNA) and peptidoglycan treatment in cultured seabream head kidney leukocytes. Using purified immune cells, sbIL-6 expression was induced similarly in macrophages and acidophilic granulocytes by
VaDNA but LPS was more effective in inducing sbIL-6 expression in acidophilic granulocytes than in macrophages. Furthermore,
in vivo infection of seabream with live
V. anguillarum caused significant increases in sbIL-6 mRNA expression in the thymus, peritoneal exudate, head kidney and gills. In summary, our study provides further evidence for the existence of distinct IL-6 genes in lower vertebrates and for the strong induction of their expression by immune stimuli, supporting the notion of a potentially important role for this cytokine in fish.</description><identifier>ISSN: 0161-5890</identifier><identifier>EISSN: 1872-9142</identifier><identifier>DOI: 10.1016/j.molimm.2008.04.012</identifier><identifier>PMID: 18513800</identifier><language>eng</language><publisher>England: Elsevier Ltd</publisher><subject>Amino Acid Sequence ; Animals ; Base Sequence ; Cloning, Molecular ; Fish ; Gene Expression Profiling ; Gene Expression Regulation ; Interleukin-6 ; Interleukin-6 - chemistry ; Interleukin-6 - genetics ; Interleukin-6 - metabolism ; Leukocytes ; Marine ; Molecular Sequence Data ; mRNA expression ; Phylogeny ; RNA, Messenger - genetics ; RNA, Messenger - metabolism ; Sea Bream - genetics ; Sea Bream - microbiology ; Sequence Alignment ; Sparus aurata ; Vibrio ; Vibrio anguillarum</subject><ispartof>Molecular immunology, 2008-07, Vol.45 (12), p.3363-3370</ispartof><rights>2008 Elsevier Ltd</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c489t-e181a8714c13fac62395d0ce92b10a35fc4b74aa3cebfc2d1f029ed413ffeebd3</citedby><cites>FETCH-LOGICAL-c489t-e181a8714c13fac62395d0ce92b10a35fc4b74aa3cebfc2d1f029ed413ffeebd3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18513800$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Castellana, Barbara</creatorcontrib><creatorcontrib>Iliev, Dimitar B.</creatorcontrib><creatorcontrib>Sepulcre, M. Pilar</creatorcontrib><creatorcontrib>MacKenzie, Simon</creatorcontrib><creatorcontrib>Goetz, Frederick W.</creatorcontrib><creatorcontrib>Mulero, Victoriano</creatorcontrib><creatorcontrib>Planas, Josep V.</creatorcontrib><title>Molecular characterization of interleukin-6 in the gilthead seabream ( Sparus aurata)</title><title>Molecular immunology</title><addtitle>Mol Immunol</addtitle><description>A cDNA clone, designated sbIL-6 (seabream interleukin-6), was obtained from a cDNA library of enriched immune-stimulated sequences from gilthead seabream. The deduced sbIL-6 protein corresponds to a 225-amino acid protein with a putative 24-amino acid signal peptide, four conserved alpha helices and one
N-linked glycosylation site. At the amino acid level sbIL-6 shares 23–26% identity with mammalian IL-6 sequences and 30–51% identity with other fish IL-6 sequences. The structure of the sbIL-6 gene consisted of 5 exons and 4 introns, spanning 2.4
kb. Healthy fish expressed sbIL-6 in white muscle, skin, spleen, anterior intestine and stomach, while no expression was detected in brain, gill, head kidney, posterior intestine and adipose tissue. A significant up-regulation of sbIL-6 expression was observed after lipopolysaccharide (LPS),
Vibrio anguillarum DNA (
VaDNA) and peptidoglycan treatment in cultured seabream head kidney leukocytes. Using purified immune cells, sbIL-6 expression was induced similarly in macrophages and acidophilic granulocytes by
VaDNA but LPS was more effective in inducing sbIL-6 expression in acidophilic granulocytes than in macrophages. Furthermore,
in vivo infection of seabream with live
V. anguillarum caused significant increases in sbIL-6 mRNA expression in the thymus, peritoneal exudate, head kidney and gills. In summary, our study provides further evidence for the existence of distinct IL-6 genes in lower vertebrates and for the strong induction of their expression by immune stimuli, supporting the notion of a potentially important role for this cytokine in fish.</description><subject>Amino Acid Sequence</subject><subject>Animals</subject><subject>Base Sequence</subject><subject>Cloning, Molecular</subject><subject>Fish</subject><subject>Gene Expression Profiling</subject><subject>Gene Expression Regulation</subject><subject>Interleukin-6</subject><subject>Interleukin-6 - chemistry</subject><subject>Interleukin-6 - genetics</subject><subject>Interleukin-6 - metabolism</subject><subject>Leukocytes</subject><subject>Marine</subject><subject>Molecular Sequence Data</subject><subject>mRNA expression</subject><subject>Phylogeny</subject><subject>RNA, Messenger - genetics</subject><subject>RNA, Messenger - metabolism</subject><subject>Sea Bream - genetics</subject><subject>Sea Bream - microbiology</subject><subject>Sequence Alignment</subject><subject>Sparus aurata</subject><subject>Vibrio</subject><subject>Vibrio anguillarum</subject><issn>0161-5890</issn><issn>1872-9142</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><recordid>eNqFkU2LFDEQhoMo7uzqPxDJyY9Dt1XpdHdyEWRxVVjxoHsO1elqN2N_jEm3oL_eLDPgbT29vPBUFdQjxDOEEgGbN_tyWsYwTaUCMCXoElA9EDs0rSosavVQ7DKGRW0snInzlPYA0EBTPxZnaGqsDMBO3HxeRvbbSFH6W4rkV47hD61hmeUyyDDnPvL2I8xFk5tcb1l-D2MO6mVi6iLTJF_JrweKW5K0RVrp9RPxaKAx8dNTXoibq_ffLj8W118-fLp8d114bexaMBok06L2WA3kG1XZugfPVnUIVNWD112riSrP3eBVjwMoy73O9MDc9dWFeHnce4jLz43T6qaQPI8jzbxsyRlTgW5rZTL54l6yxaax1v4fVGBbbbTOoD6CPi4pRR7cIYaJ4m-H4O4Mub07GnJ3hhxolw3lseen_Vs3cf9v6KQkA2-PAOfH_QocXfKBZ899iOxX1y_h_gt_AVL0pGw</recordid><startdate>20080701</startdate><enddate>20080701</enddate><creator>Castellana, Barbara</creator><creator>Iliev, Dimitar B.</creator><creator>Sepulcre, M. 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Pilar ; MacKenzie, Simon ; Goetz, Frederick W. ; Mulero, Victoriano ; Planas, Josep V.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c489t-e181a8714c13fac62395d0ce92b10a35fc4b74aa3cebfc2d1f029ed413ffeebd3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>Amino Acid Sequence</topic><topic>Animals</topic><topic>Base Sequence</topic><topic>Cloning, Molecular</topic><topic>Fish</topic><topic>Gene Expression Profiling</topic><topic>Gene Expression Regulation</topic><topic>Interleukin-6</topic><topic>Interleukin-6 - chemistry</topic><topic>Interleukin-6 - genetics</topic><topic>Interleukin-6 - metabolism</topic><topic>Leukocytes</topic><topic>Marine</topic><topic>Molecular Sequence Data</topic><topic>mRNA expression</topic><topic>Phylogeny</topic><topic>RNA, Messenger - genetics</topic><topic>RNA, Messenger - metabolism</topic><topic>Sea Bream - genetics</topic><topic>Sea Bream - microbiology</topic><topic>Sequence Alignment</topic><topic>Sparus aurata</topic><topic>Vibrio</topic><topic>Vibrio anguillarum</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Castellana, Barbara</creatorcontrib><creatorcontrib>Iliev, Dimitar B.</creatorcontrib><creatorcontrib>Sepulcre, M. Pilar</creatorcontrib><creatorcontrib>MacKenzie, Simon</creatorcontrib><creatorcontrib>Goetz, Frederick W.</creatorcontrib><creatorcontrib>Mulero, Victoriano</creatorcontrib><creatorcontrib>Planas, Josep V.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Immunology Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Aquaculture Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><jtitle>Molecular immunology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Castellana, Barbara</au><au>Iliev, Dimitar B.</au><au>Sepulcre, M. Pilar</au><au>MacKenzie, Simon</au><au>Goetz, Frederick W.</au><au>Mulero, Victoriano</au><au>Planas, Josep V.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Molecular characterization of interleukin-6 in the gilthead seabream ( Sparus aurata)</atitle><jtitle>Molecular immunology</jtitle><addtitle>Mol Immunol</addtitle><date>2008-07-01</date><risdate>2008</risdate><volume>45</volume><issue>12</issue><spage>3363</spage><epage>3370</epage><pages>3363-3370</pages><issn>0161-5890</issn><eissn>1872-9142</eissn><abstract>A cDNA clone, designated sbIL-6 (seabream interleukin-6), was obtained from a cDNA library of enriched immune-stimulated sequences from gilthead seabream. The deduced sbIL-6 protein corresponds to a 225-amino acid protein with a putative 24-amino acid signal peptide, four conserved alpha helices and one
N-linked glycosylation site. At the amino acid level sbIL-6 shares 23–26% identity with mammalian IL-6 sequences and 30–51% identity with other fish IL-6 sequences. The structure of the sbIL-6 gene consisted of 5 exons and 4 introns, spanning 2.4
kb. Healthy fish expressed sbIL-6 in white muscle, skin, spleen, anterior intestine and stomach, while no expression was detected in brain, gill, head kidney, posterior intestine and adipose tissue. A significant up-regulation of sbIL-6 expression was observed after lipopolysaccharide (LPS),
Vibrio anguillarum DNA (
VaDNA) and peptidoglycan treatment in cultured seabream head kidney leukocytes. Using purified immune cells, sbIL-6 expression was induced similarly in macrophages and acidophilic granulocytes by
VaDNA but LPS was more effective in inducing sbIL-6 expression in acidophilic granulocytes than in macrophages. Furthermore,
in vivo infection of seabream with live
V. anguillarum caused significant increases in sbIL-6 mRNA expression in the thymus, peritoneal exudate, head kidney and gills. In summary, our study provides further evidence for the existence of distinct IL-6 genes in lower vertebrates and for the strong induction of their expression by immune stimuli, supporting the notion of a potentially important role for this cytokine in fish.</abstract><cop>England</cop><pub>Elsevier Ltd</pub><pmid>18513800</pmid><doi>10.1016/j.molimm.2008.04.012</doi><tpages>8</tpages></addata></record> |
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subjects | Amino Acid Sequence Animals Base Sequence Cloning, Molecular Fish Gene Expression Profiling Gene Expression Regulation Interleukin-6 Interleukin-6 - chemistry Interleukin-6 - genetics Interleukin-6 - metabolism Leukocytes Marine Molecular Sequence Data mRNA expression Phylogeny RNA, Messenger - genetics RNA, Messenger - metabolism Sea Bream - genetics Sea Bream - microbiology Sequence Alignment Sparus aurata Vibrio Vibrio anguillarum |
title | Molecular characterization of interleukin-6 in the gilthead seabream ( Sparus aurata) |
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