Endocrine profiles of somatic nuclear transfer-derived pregnancies in dairy cattle

In cattle, several hormones and proteins are necessary for maintenance of a normal pregnancy that will result in a viable calf. Deviation from the normal cascade or expected profile of reproductive hormones and proteins may be associated with impairment of somatic nuclear transfer-derived pregnancie...

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Bibliographic Details
Published in:Theriogenology 2011-09, Vol.76 (5), p.911-920
Main Authors: Kohan-Ghadr, H.R, Fecteau, G, Smith, L.C, Murphy, B.D, Lefebvre, R.C
Format: Article
Language:English
Subjects:
Animals
Bovine
calves
Cattle - blood
Clone
Cloning, Organism - methods
Cloning, Organism - veterinary
cows
dairy cattle
embryo transfer
Endocrine hormones
estradiol
Estradiol - blood
estrone
Estrone - analogs & derivatives
Estrone - blood
estrus
Female
fetal death
Gestational Age
Monitoring
Nuclear Transfer Techniques - veterinary
Pregnancy
Pregnancy Proteins - blood
Pregnancy-Specific beta 1-Glycoproteins - analysis
progesterone
Progesterone - blood
proteins
ultrasonography
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Summary:In cattle, several hormones and proteins are necessary for maintenance of a normal pregnancy that will result in a viable calf. Deviation from the normal cascade or expected profile of reproductive hormones and proteins may be associated with impairment of somatic nuclear transfer-derived pregnancies and the high rate of fetal loss. The objectives of this study were to characterize maternal plasma concentrations of pregnancy-specific protein B (PSPB), progesterone (P4), estrone sulphate (E₁S), and estradiol (E2) during the last two-thirds of pregnancy (cloned calves), and to determine associations with gestational abnormalities. Cows with cloned fetuses, produced by either commercial (N = 16) or zona-free (N = 4) cloning techniques, were compared with pregnant animals derived from traditional embryo transfer (N = 6) or AI (N = 6), at various stages of gestation (Days 80, 120, 150, 180, 210, and 240; Day 0 = estrus). Fetal well-being was monitored with ultrasonography throughout gestation. At Day 80, progesterone concentration was lower (P < 0.0001) in nuclear transfer (NT) recipients than in control groups. Mean estrone sulphate concentrations did not vary significantly between NT and control groups. At Day 150, pregnancy-specific protein B concentrations were elevated (P < 0.002) in NT cows. Estradiol concentration was higher in NT recipients than control cows throughout the study period.
ISSN:0093-691X
1879-3231
DOI:10.1016/j.theriogenology.2011.04.022