Effect of Microfluidization on in Vitro Micellization and Intestinal Cell Uptake of Lutein from Chlorella vulgaris

Chlorella is a nutrient-rich microalga that contains protein, lipid, minerals, vitamins, and high levels of lutein. This study evaluated the bioavailability of lutein from Chlorella vulgaris using a coupled in vitro digestion and human intestinal Caco-2 cell model. Lutein bioaccessibility was low, a...

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Bibliographic Details
Published in:Journal of agricultural and food chemistry 2011-08, Vol.59 (16), p.8670-8674
Main Authors: Cha, Kwang Hyun, Lee, Joo Young, Song, Dae-Geun, Kim, Sang Min, Lee, Dong-Un, Jeon, Jin-Young, Pan, Cheol-Ho
Format: Article
Language:English
Subjects:
Bioactive Constituents
Biological and medical sciences
Biological Availability
Caco-2 Cells
Cell Wall - metabolism
Chlorella vulgaris - chemistry
Chlorella vulgaris - ultrastructure
Digestion
Food Handling - methods
Food industries
Fundamental and applied biological sciences. Psychology
Humans
Intestinal Absorption
Lutein - metabolism
Lutein - pharmacokinetics
Micelles
Microfluidics
Microscopy, Electron, Scanning
Models, Biological
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Summary:Chlorella is a nutrient-rich microalga that contains protein, lipid, minerals, vitamins, and high levels of lutein. This study evaluated the bioavailability of lutein from Chlorella vulgaris using a coupled in vitro digestion and human intestinal Caco-2 cell model. Lutein bioaccessibility was low, and approximately 75% of total C. vulgaris lutein was not micellized during the digestion process but remained in the insoluble digestate. Microfluidization improved lutein micellization efficiency during C. vulgaris digestion. C. vulgaris was microfluidized at a pressure exceeding 10000 psi, and the cell surface disruption was visualized by scanning electron microscopy. The mean C. vulgaris particle size was reduced from 3.56 to 0.35 μm with the microfluidization treatment. C. vulgaris microfluidization at 20000 psi was three times more efficient for aqueous lutein micelles production as compared with untreated C. vulgaris, and the final lutein content accumulated by intestinal Caco-2 cells was also higher with microfluidization. C. vulgaris lutein stability was not affected by microfluidization. These results indicate that microfluidization may be useful for improving lutein bioaccessibility from C. vulgaris during food processing.
ISSN:0021-8561
1520-5118
DOI:10.1021/jf2019243