Naturally appearing N-feruloylserotonin isomers suppress oxidative burst of human neutrophils at the protein kinase C level

• Published in: Pharmacological reports 2011, Vol.63 (3), p.790-798
• Main Authors: Nosál’, Rado, Pereěko, Tomáš, Jančinová, Viera, Drábiková, Katarína, Harmatha, Juraj, Sviteková, Klara
• Format: Article
• Language: English
• Subjects: Adult; Cell Survival - drug effects; Dose-Response Relationship, Drug; Drug Safety and Pharmacovigilance; extracellular and intracellular chemiluminescence; Humans; Isomerism; Leuzea - chemistry; Luminescent Measurements; Male; Middle Aged; N-feruloylserotonin; neutrophils; Neutrophils - drug effects; Neutrophils - metabolism; Pharmacotherapy; Pharmacy; Phosphorylation - drug effects; Protein Kinase C - metabolism; protein kinase C phosphorylation; Reactive Oxygen Species - metabolism; Respiratory Burst - drug effects; Seeds; Serotonin - analogs & derivatives; Serotonin - chemistry; Serotonin - isolation & purification; Serotonin - pharmacology; Tetradecanoylphorbol Acetate - pharmacology; Young Adult
• ISSN: 1734-1140; 2299-5684
• DOI: 10.1016/S1734-1140(11)70591-6

N-feruloylserotonin (N-f-5HT) isomers, isolated from seeds of Leuzea carthamoides (Wild) DC, inhibited dose-dependent oxidative burst in human whole blood and isolated neutrophils in vitro, which were measured by luminol- and/or isoluminol-enhanced chemiluminescence in the following rank order of stimuli: PMA > OpZ > calcium ionophore A23187. In isolated neutrophils that were stimulated with PMA, N-f-5HT isomers were effective against extracellular and intracellular reactive oxygen species. Liberation of ATP, analysis of apoptosis, and recombinant caspase-3 activity revealed that N-f-5HT isomers, used in concentrations up to 100µM, did not alter the viability and integrity of isolated neutrophils. Western blot analysis documented that in concentrations of 10 and 100µM, N-f-5HT isomers significantly decreased PMA-induced phosphorylation of PKC α/β II. The results suggest that N-f-5HT isomers are an effective, naturally occurring substance with a potent pharmacological effect on the oxidative burst of human neutrophils. It should be further investigated for its pharmacological activity against oxidative stress in ischemia-reperfusion, inflammation and other pathological conditions.