Effects of daily administration of a stavudine/nelfinavir association on the fetuses and placentas of female albino rats

• Published in: Revista Brasileira de ginecologia e obstetrícia 2011-05, Vol.33 (5), p.225-230
• Main Authors: de Carvalho, Gisela Maria Vieira Rodrigues, Nakamura, Mary Uchiyama, Simões, Ricardo Santos, Antonio, Eliana Maria Restum, Wagngner, Adriana, Fontes, Tereza Maria Pereira, Kulay Júnior, Luiz
• Format: Article
• Language: Portuguese
• Subjects: Animals; Anti-HIV Agents - administration & dosage; Drug Administration Schedule; Drug Therapy, Combination; Female; Fetus - drug effects; Nelfinavir - administration & dosage; Placenta - drug effects; Pregnancy; Rats; Rats, Wistar; Stavudine - administration & dosage
• ISSN: 1806-9339

To evaluate the effect of administration of a stavudine/nelfinavir combination on the rat pregnancy by assessing maternal and concepts weights, as well as the number of implantations, fetuses, placentas, resorptions and maternal and fetal mortality. Forty adult pregnant Wistar rats of the EPM-1 strain were randomly divided into four groups: control (GCtrl--drug vehicle control, n=10), and three experimental groups, which were treated with an oral solution of stavudine/nelfinavir (ExpI--1/40 mg/kg b.w., n=10; ExpII--3/120 mg/kg b.w., n=10; ExpIII--9/360 mg/kg b.w., n=10) from day 0 to the 20th day of pregnancy. Maternal body weights were determined at the start of the experiment and on the 7th, 14th and the 20th day thereafter. At term (20th day) the rats were anesthetized and, upon laparotomy and hysterotomy, the number of implantations, resorptions, living fetuses, placentae and intrauterine deaths were recorded. The collected fetuses and placentae were weighed and the concepts were examined under a stereomicroscope for possible external malformations. Statistical analysis was performed by analysis of variance (ANOVA) complemented by the Kruskal-Wallis test (p