Steady-state bioluminescence of bacterial luciferase using electrochemical regeneration of flavin substrate and its application to inhibitory analysis

The model system for the biological reaction using a bacterial luciferase (BL) was developed and applied to the inhibitory analysis of the hydrophobic molecules for enzymatic reactions. The homemade flow electrochemical luminescence cell was embedded in the BL reaction system to regenerate the reduc...

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Bibliographic Details
Published in:Bioelectrochemistry (Amsterdam, Netherlands) Netherlands), 2009-04, Vol.75 (1), p.67-70
Main Authors: Yamasaki, Shinya, Nakashima, Shinya, Yamada, Shuto, Takehara, Kô
Format: Article
Language:English
Subjects:
Aliivibrio fischeri - enzymology
Amides - pharmacology
Bacteria
Bacterial luciferase
Biosensing Techniques - methods
Continuous luminescence
Electrochemical reduction
Electrochemical Techniques - methods
Flavins - chemistry
Flavins - metabolism
Luciferases - antagonists & inhibitors
Luciferases - metabolism
Luminescent Measurements - methods
Substrate Specificity
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Summary:The model system for the biological reaction using a bacterial luciferase (BL) was developed and applied to the inhibitory analysis of the hydrophobic molecules for enzymatic reactions. The homemade flow electrochemical luminescence cell was embedded in the BL reaction system to regenerate the reduced form of the flavin mononucleotide, which is one of the substrates of the BL luminescence reaction, and to measure the luminescence intensity. The constant intensity of the continuous BL luminescence was observed using the continuous-flow BL reaction system. The proposed system was successfully applied to the inhibitory reaction of dodecaneamide on the BL luminescence reaction.
ISSN:1567-5394
1878-562X
DOI:10.1016/j.bioelechem.2008.12.002