Construction of Multidrug-Sensitive Yeast with High Sporulation Efficiency

• Published in: Bioscience, biotechnology, and biochemistry biotechnology, and biochemistry, 2011, Vol.75 (8), p.1588-1593
• Main Authors: CHINEN, Takumi, OTA, Yu, NAGUMO, Yoko, MASUMOTO, Hiroshi, USUI, Takeo
• Format: Article
• Language: English
• Subjects: Antifungal Agents - pharmacology; ATP-binding cassette (ABC) transporter; Biological and medical sciences; chemical genetics; Fundamental and applied biological sciences. Psychology; Gene Deletion; Gene Expression Regulation, Fungal; Genetic Engineering - methods; Inhibitory Concentration 50; Microbial Sensitivity Tests; Multidrug Resistance-Associated Proteins - deficiency; Multidrug Resistance-Associated Proteins - genetics; multidrug-sensitive; Mutation; Organisms, Genetically Modified - genetics; Organisms, Genetically Modified - metabolism; Plasmids; Saccharomyces cerevisiae - drug effects; Saccharomyces cerevisiae - genetics; Saccharomyces cerevisiae - metabolism; transcription factor; Transcription Factors - deficiency; Transcription Factors - genetics; Transduction, Genetic
• ISSN: 0916-8451; 1347-6947
• DOI: 10.1271/bbb.110311

Budding yeast is often used in chemical genetics for screening, target identification, and compound verification, but its high-level drug resistance has made the analysis of compounds difficult. Here we report the construction of 12geneΔ0HSR, a strain that lacks eight efflux pumps located on the plasma membrane and four transcription factors involved in expression of efflux pumps, and contains the RME1(ins-308A) mutation. This strain retained sufficient transformation, mating, and sporulation efficiency for genetic analysis in addition to hypersensitivity against several compounds. 12geneΔ0HSR is a useful tool for chemical biology, not only in chemical screening but in target identification and verification of bioactive compounds.