Assessment of HB-EGF Levels in Peritoneal Fluid and Serum of Ovarian Cancer Patients using ELISA

Heparin-binding epidermal growth factor-like growth factor (HB-EGF) is a rational target for ovarian cancer therapy. The aim of this study was to examine HB-EGF levels in the peritoneal fluid and serum of ovarian cancer (OVCA) patients. Samples were collected from six healthy women, 21 OVCA patients...

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Bibliographic Details
Published in:Anticancer research 2011-07, Vol.31 (7), p.2553-2559
Main Authors: HIKITA, Shoko, YOTSUMOTO, Fusanori, YOSHIZATO, Toshiyuki, MAEDA, Kazuhiro, ISHIKAWA, Toyokazu, OKUNO, Yoshinobu, KUROKI, Masahide, MEKADA, Eisuke, MIYAMOTO, Shingo, FUMAKI, Tatsuya, HORIUCHI, Shinji, SANUI, Ayako, MIYATA, Kohei, SUNG OUK NAM, TSUJIOKA, Hiroshi, UEDA, Taeko, SHIROTA, Kyoko
Format: Article
Language:English
Subjects:
Adult
Aged
Antibodies - immunology
Antibody Affinity
Antibody Specificity
Ascitic Fluid - chemistry
Binding, Competitive
Biological and medical sciences
Biomarkers, Tumor
Enzyme-Linked Immunosorbent Assay
Female
Female genital diseases
Gynecology. Andrology. Obstetrics
Heparin-binding EGF-like Growth Factor
Humans
Intercellular Signaling Peptides and Proteins - analysis
Intercellular Signaling Peptides and Proteins - blood
Intercellular Signaling Peptides and Proteins - immunology
Medical sciences
Middle Aged
Neoplasm Proteins - analysis
Neoplasm Proteins - blood
Neoplasm Proteins - immunology
Ovarian Cysts - blood
Ovarian Cysts - metabolism
Ovarian Neoplasms - blood
Ovarian Neoplasms - metabolism
Protein Binding
Reproducibility of Results
Sensitivity and Specificity
Tumors
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Summary:Heparin-binding epidermal growth factor-like growth factor (HB-EGF) is a rational target for ovarian cancer therapy. The aim of this study was to examine HB-EGF levels in the peritoneal fluid and serum of ovarian cancer (OVCA) patients. Samples were collected from six healthy women, 21 OVCA patients, and 21 ovarian cyst patients. HB-EGF levels were measured using a sandwich ELISA kit and calculated using a parallel line assay. No significant difference between the slopes of the standard and sample curves was observed at an anti-HB-EGF antibody concentration of 1.6 μg/ml. HB-EGF levels in the peritoneal fluid and serum of OVCA patients were significantly higher than those in patients with ovarian cysts or controls. Serum HB-EGF levels were also significantly correlated with levels in peritoneal fluid in OVCA patients. We developed an assay for the exact measurement of HB-EGF levels in peritoneal fluid and serum.
ISSN:0250-7005
1791-7530