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The trophoblast binucleate cell is the source of maternal circulating C-type natriuretic peptide during ovine pregnancy

Abstract Maternal plasma concentrations of C-type natriuretic peptide (CNP) and a co-secreted bioinactive amino-terminal fragment (NTproCNP) are elevated during ovine pregnancy. Although the uteroplacental unit has been implicated as a likely source of CNP, the relative contributions of specific ute...

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Published in:	Placenta (Eastbourne) 2011-09, Vol.32 (9), p.645-650
Main Authors:	McNeill, B.A, Barrell, G.K, Wooding, F.B.P, Prickett, T.C.R, Espiner, E.A
Format:	Article
Language:	English
Subjects:	Animals Binucleate cell Biological and medical sciences C-type natriuretic peptide Embryology: invertebrates and vertebrates. Teratology Female Fetus Fundamental and applied biological sciences. Psychology Internal Medicine Natriuretic Peptide, C-Type - blood NTproCNP Obstetrics and Gynecology Placenta Placenta - metabolism Pregnancy Pregnancy, Animal - physiology Protein Precursors - blood Sheep Sheep, Domestic Trophoblasts - cytology Trophoblasts - metabolism Uterus - metabolism
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creator	McNeill, B.A Barrell, G.K Wooding, F.B.P Prickett, T.C.R Espiner, E.A
description	Abstract Maternal plasma concentrations of C-type natriuretic peptide (CNP) and a co-secreted bioinactive amino-terminal fragment (NTproCNP) are elevated during ovine pregnancy. Although the uteroplacental unit has been implicated as a likely source of CNP, the relative contributions of specific uterine and placental tissues, and identity of the cellular site/s of production remain unknown. Therefore, we measured CNP and NTproCNP in intercaruncular uterine tissue and maternal (caruncle) and fetal (cotyledon) placental tissues throughout gestation. Concentrations of CNP forms in placental tissues greatly exceeded those in intercaruncular uterine tissue throughout pregnancy ( P
doi_str_mv	10.1016/j.placenta.2011.06.001
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Although the uteroplacental unit has been implicated as a likely source of CNP, the relative contributions of specific uterine and placental tissues, and identity of the cellular site/s of production remain unknown. Therefore, we measured CNP and NTproCNP in intercaruncular uterine tissue and maternal (caruncle) and fetal (cotyledon) placental tissues throughout gestation. Concentrations of CNP forms in placental tissues greatly exceeded those in intercaruncular uterine tissue throughout pregnancy ( P < 0.05). Mean caruncular concentrations (CNP 32 ± 4, NTproCNP 56 ± 6 pmol g−1 ) peaked at day 60 whereas in the cotyledon there was a progressive increase in CNP forms to peak values (CNP 66 ± 6, NTproCNP 134 ± 9 pmol g−1 ) at day 100–135 followed by a sharp decline just prior to term (day 143). At term CNP gene expression was 6-fold greater in placental tissue compared with intercaruncular uterine tissue. Changes in maternal plasma concentration of CNP forms closely followed those in cotyledonary tissue whereas fetal plasma levels fell progressively throughout gestation. Immunohistochemistry revealed staining in binucleate cells (BNC) and around placental blood vessels. CNP’s localization to the BNC suggests a novel endocrine role during pregnancy, in addition to its paracrine actions within the placental vasculature. The function of CNP in maternal circulation remains to be determined, but as proposed for other BNC products, may involve manipulation of maternal physiology and placental function to favour fetal growth.</description><identifier>ISSN: 0143-4004</identifier><identifier>EISSN: 1532-3102</identifier><identifier>DOI: 10.1016/j.placenta.2011.06.001</identifier><identifier>PMID: 21705079</identifier><identifier>CODEN: PLACDF</identifier><language>eng</language><publisher>Kidlington: Elsevier Ltd</publisher><subject>Animals ; Binucleate cell ; Biological and medical sciences ; C-type natriuretic peptide ; Embryology: invertebrates and vertebrates. Teratology ; Female ; Fetus ; Fundamental and applied biological sciences. Psychology ; Internal Medicine ; Natriuretic Peptide, C-Type - blood ; NTproCNP ; Obstetrics and Gynecology ; Placenta ; Placenta - metabolism ; Pregnancy ; Pregnancy, Animal - physiology ; Protein Precursors - blood ; Sheep ; Sheep, Domestic ; Trophoblasts - cytology ; Trophoblasts - metabolism ; Uterus - metabolism</subject><ispartof>Placenta (Eastbourne), 2011-09, Vol.32 (9), p.645-650</ispartof><rights>Elsevier Ltd</rights><rights>2011 Elsevier Ltd</rights><rights>2015 INIST-CNRS</rights><rights>Copyright © 2011 Elsevier Ltd. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c452t-35a15d593406cbd85b50a15eaffd8cf6c28e1180cd5f903ce98c4bca98eeef613</citedby><cites>FETCH-LOGICAL-c452t-35a15d593406cbd85b50a15eaffd8cf6c28e1180cd5f903ce98c4bca98eeef613</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=24492706$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21705079$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>McNeill, B.A</creatorcontrib><creatorcontrib>Barrell, G.K</creatorcontrib><creatorcontrib>Wooding, F.B.P</creatorcontrib><creatorcontrib>Prickett, T.C.R</creatorcontrib><creatorcontrib>Espiner, E.A</creatorcontrib><title>The trophoblast binucleate cell is the source of maternal circulating C-type natriuretic peptide during ovine pregnancy</title><title>Placenta (Eastbourne)</title><addtitle>Placenta</addtitle><description>Abstract Maternal plasma concentrations of C-type natriuretic peptide (CNP) and a co-secreted bioinactive amino-terminal fragment (NTproCNP) are elevated during ovine pregnancy. Although the uteroplacental unit has been implicated as a likely source of CNP, the relative contributions of specific uterine and placental tissues, and identity of the cellular site/s of production remain unknown. Therefore, we measured CNP and NTproCNP in intercaruncular uterine tissue and maternal (caruncle) and fetal (cotyledon) placental tissues throughout gestation. Concentrations of CNP forms in placental tissues greatly exceeded those in intercaruncular uterine tissue throughout pregnancy ( P < 0.05). Mean caruncular concentrations (CNP 32 ± 4, NTproCNP 56 ± 6 pmol g−1 ) peaked at day 60 whereas in the cotyledon there was a progressive increase in CNP forms to peak values (CNP 66 ± 6, NTproCNP 134 ± 9 pmol g−1 ) at day 100–135 followed by a sharp decline just prior to term (day 143). At term CNP gene expression was 6-fold greater in placental tissue compared with intercaruncular uterine tissue. Changes in maternal plasma concentration of CNP forms closely followed those in cotyledonary tissue whereas fetal plasma levels fell progressively throughout gestation. Immunohistochemistry revealed staining in binucleate cells (BNC) and around placental blood vessels. CNP’s localization to the BNC suggests a novel endocrine role during pregnancy, in addition to its paracrine actions within the placental vasculature. The function of CNP in maternal circulation remains to be determined, but as proposed for other BNC products, may involve manipulation of maternal physiology and placental function to favour fetal growth.</description><subject>Animals</subject><subject>Binucleate cell</subject><subject>Biological and medical sciences</subject><subject>C-type natriuretic peptide</subject><subject>Embryology: invertebrates and vertebrates. Teratology</subject><subject>Female</subject><subject>Fetus</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Internal Medicine</subject><subject>Natriuretic Peptide, C-Type - blood</subject><subject>NTproCNP</subject><subject>Obstetrics and Gynecology</subject><subject>Placenta</subject><subject>Placenta - metabolism</subject><subject>Pregnancy</subject><subject>Pregnancy, Animal - physiology</subject><subject>Protein Precursors - blood</subject><subject>Sheep</subject><subject>Sheep, Domestic</subject><subject>Trophoblasts - cytology</subject><subject>Trophoblasts - metabolism</subject><subject>Uterus - metabolism</subject><issn>0143-4004</issn><issn>1532-3102</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><recordid>eNqFkktv1DAQgCMEokvhL1S-IE4JYyfOOhcEWvGSKnGgnC1nMmm9eJ1gO6323-NotyBx4WTJ_ubhb6YorjhUHHj7dl_NziD5ZCoBnFfQVgD8SbHhshZlzUE8LTbAm7psAJqL4kWMewDoGi6eFxeCb0HCttsUDzd3xFKY5rupdyYm1lu_oCOTiCE5x2xkKSNxWgISm0Z2yE_BG8fQBlycSdbfsl2ZjjMxb1KwS6Bkkc00JzsQG5awEtO99cTmQLfeeDy-LJ6NxkV6dT4vix-fPt7svpTX3z5_3X24LrGRIpW1NFwOsqsbaLEflOwl5Bsy4zgoHFsUijhXgIMcO6iROoVNj6ZTRDS2vL4s3pzyzmH6tVBM-mDj-jHjaVqiVqqVnVS1zGR7IjFMMQYa9RzswYSj5qBX53qvH53r1bmGVmfnOfDqXGLpDzT8CXuUnIHXZ8BENG4MWYCNf7mm6cQW2sy9P3GUhdxbCjqiJY802ECY9DDZ__fy7p8U6Ky3uepPOlLc5ynm0UXNdRQa9Pd1Q9YF4RxAQKfq3zAQurg</recordid><startdate>20110901</startdate><enddate>20110901</enddate><creator>McNeill, B.A</creator><creator>Barrell, G.K</creator><creator>Wooding, F.B.P</creator><creator>Prickett, T.C.R</creator><creator>Espiner, E.A</creator><general>Elsevier Ltd</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20110901</creationdate><title>The trophoblast binucleate cell is the source of maternal circulating C-type natriuretic peptide during ovine pregnancy</title><author>McNeill, B.A ; Barrell, G.K ; Wooding, F.B.P ; Prickett, T.C.R ; Espiner, E.A</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c452t-35a15d593406cbd85b50a15eaffd8cf6c28e1180cd5f903ce98c4bca98eeef613</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Animals</topic><topic>Binucleate cell</topic><topic>Biological and medical sciences</topic><topic>C-type natriuretic peptide</topic><topic>Embryology: invertebrates and vertebrates. Teratology</topic><topic>Female</topic><topic>Fetus</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Internal Medicine</topic><topic>Natriuretic Peptide, C-Type - blood</topic><topic>NTproCNP</topic><topic>Obstetrics and Gynecology</topic><topic>Placenta</topic><topic>Placenta - metabolism</topic><topic>Pregnancy</topic><topic>Pregnancy, Animal - physiology</topic><topic>Protein Precursors - blood</topic><topic>Sheep</topic><topic>Sheep, Domestic</topic><topic>Trophoblasts - cytology</topic><topic>Trophoblasts - metabolism</topic><topic>Uterus - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>McNeill, B.A</creatorcontrib><creatorcontrib>Barrell, G.K</creatorcontrib><creatorcontrib>Wooding, F.B.P</creatorcontrib><creatorcontrib>Prickett, T.C.R</creatorcontrib><creatorcontrib>Espiner, E.A</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Placenta (Eastbourne)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>McNeill, B.A</au><au>Barrell, G.K</au><au>Wooding, F.B.P</au><au>Prickett, T.C.R</au><au>Espiner, E.A</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The trophoblast binucleate cell is the source of maternal circulating C-type natriuretic peptide during ovine pregnancy</atitle><jtitle>Placenta (Eastbourne)</jtitle><addtitle>Placenta</addtitle><date>2011-09-01</date><risdate>2011</risdate><volume>32</volume><issue>9</issue><spage>645</spage><epage>650</epage><pages>645-650</pages><issn>0143-4004</issn><eissn>1532-3102</eissn><coden>PLACDF</coden><abstract>Abstract Maternal plasma concentrations of C-type natriuretic peptide (CNP) and a co-secreted bioinactive amino-terminal fragment (NTproCNP) are elevated during ovine pregnancy. Although the uteroplacental unit has been implicated as a likely source of CNP, the relative contributions of specific uterine and placental tissues, and identity of the cellular site/s of production remain unknown. Therefore, we measured CNP and NTproCNP in intercaruncular uterine tissue and maternal (caruncle) and fetal (cotyledon) placental tissues throughout gestation. Concentrations of CNP forms in placental tissues greatly exceeded those in intercaruncular uterine tissue throughout pregnancy ( P < 0.05). Mean caruncular concentrations (CNP 32 ± 4, NTproCNP 56 ± 6 pmol g−1 ) peaked at day 60 whereas in the cotyledon there was a progressive increase in CNP forms to peak values (CNP 66 ± 6, NTproCNP 134 ± 9 pmol g−1 ) at day 100–135 followed by a sharp decline just prior to term (day 143). At term CNP gene expression was 6-fold greater in placental tissue compared with intercaruncular uterine tissue. Changes in maternal plasma concentration of CNP forms closely followed those in cotyledonary tissue whereas fetal plasma levels fell progressively throughout gestation. Immunohistochemistry revealed staining in binucleate cells (BNC) and around placental blood vessels. CNP’s localization to the BNC suggests a novel endocrine role during pregnancy, in addition to its paracrine actions within the placental vasculature. The function of CNP in maternal circulation remains to be determined, but as proposed for other BNC products, may involve manipulation of maternal physiology and placental function to favour fetal growth.</abstract><cop>Kidlington</cop><pub>Elsevier Ltd</pub><pmid>21705079</pmid><doi>10.1016/j.placenta.2011.06.001</doi><tpages>6</tpages></addata></record>
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subjects	Animals Binucleate cell Biological and medical sciences C-type natriuretic peptide Embryology: invertebrates and vertebrates. Teratology Female Fetus Fundamental and applied biological sciences. Psychology Internal Medicine Natriuretic Peptide, C-Type - blood NTproCNP Obstetrics and Gynecology Placenta Placenta - metabolism Pregnancy Pregnancy, Animal - physiology Protein Precursors - blood Sheep Sheep, Domestic Trophoblasts - cytology Trophoblasts - metabolism Uterus - metabolism
title	The trophoblast binucleate cell is the source of maternal circulating C-type natriuretic peptide during ovine pregnancy
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