Phylogenetic Diversity and Metabolic Potential of Activated Sludge Microbial Communities in Full-Scale Wastewater Treatment Plants

• Published in: Environmental science & technology 2011-09, Vol.45 (17), p.7408-7415
• Main Authors: Yang, Chao, Zhang, Wei, Liu, Ruihua, Li, Qiang, Li, Baobin, Wang, Shufang, Song, Cunjiang, Qiao, Chuanling, Mulchandani, Ashok
• Format: Article
• Language: English
• Subjects: Applied sciences; Bacteria - classification; Bacteria - genetics; Bacteria - metabolism; Biological and medical sciences; Biological treatment of waters; Bioreactors - microbiology; Biotechnology; Carbon - chemistry; Carbon - metabolism; China; Environment and pollution; Exact sciences and technology; Fundamental and applied biological sciences. Psychology; General purification processes; Gram-negative bacteria; Industrial applications and implications. Economical aspects; Metabolism; Phylogenetics; Phylogeny; Pollution; Polymorphism; Polymorphism, Restriction Fragment Length; Proteobacteria - classification; Proteobacteria - genetics; Proteobacteria - metabolism; Remediation and Control Technologies; RNA, Ribosomal, 16S - analysis; RNA, Ribosomal, 16S - genetics; Sequence Analysis, DNA; Sewage - chemistry; Sewage - microbiology; Sludge; Waste Disposal, Fluid - methods; Wastewaters; Water treatment; Water treatment and pollution; Water treatment plants
• ISSN: 0013-936X; 1520-5851
• DOI: 10.1021/es2010545

The activated sludge process is an essential process for treating domestic and industrial wastewaters in most wastewater treatment plants (WWTPs). This process consists of a mixture of general and special microorganisms in a form of a complex enrichment population. Thus, the exploration of activated sludge microbial communities is crucial to improve the performance of activated sludge process. In this study, we investigated the phylogenetic diversity and metabolic potential of activated sludge microbial communities in full-scale WWTPs. Four 16S rRNA gene clone libraries were constructed from activated sludge samples. In all samples, Proteobacteria was the most abundant phylogenetic group, followed by Bacteroidetes and Firmicutes. The dominance of Proteobacteria was further demonstrated by denaturing gradient gel electrophoresis (DGGE) and terminal restriction fragment length polymorphism (T-RFLP). Some specific genera, e.g., Nitrosomonas, Thauera, and Dechloromonas, which significantly correlate with the functions and performance of wastewater treatment, were abundant in all samples. A large number of unclassified sequences were found in the library, suggesting that a wide variety of novel species may inhabit complex activated sludge communities. The structures of the bacterial community did not differ significantly among samples. All samples utilized the vast majority of 31 carbon sources of an EcoPlate (Biolog), suggesting that activated sludge microbial communities possess high metabolic potential and equivalent functions required for wastewater treatment.