Design and Characterization of a Two-Stage Human Subject Exposure Chamber

• Published in: Journal of the Air & Waste Management Association (1995) 2011-08, Vol.61 (8), p.864-871
• Main Authors: Kuprov, Roman Y., Buck, David, Pope, C. Arden, Eatough, Delbert J., Hansen, Jaron C.
• Format: Article
• Language: English
• Subjects: Adult; Aerosols; Air pollution; Applied sciences; Atmosphere Exposure Chambers; Atmospheric conditions; Atmospheric pollution; Carbon dioxide; Carbon dioxide concentration; Carbon monoxide; Cerebrovascular system; Chemical engineering; Coal; Combustion; Combustion chambers; Combustion products; Design; Design and construction; Disease; Electron microscopy; Emissions; Engineering design; Environmental testing; Equipment and supplies; Equipment Design; Exact sciences and technology; Experiments; Exposure; Gases; Health problems; Heart attacks; Human subjects; Humans; Lamps; Methods; Microscopy, Electron, Scanning; Morphology; Nitric oxide; Nitrogen dioxide; Oxidation; Ozone; Particle Size; Particulate emissions; Particulate matter; Particulate Matter - adverse effects; Photochemicals; Pollutants; Pollution; Pollution monitoring; Polytetrafluoroethylene; Reactors; Scanning electron microscopy; Smoke; Standard deviation; Studies; Testing equipment; Wood; X-ray fluorescence
• ISSN: 1096-2247; 2162-2906
• DOI: 10.3155/1047-3289.61.8.864

A human subject exposure chamber, designed to hold six to eight subjects, coupled to an approximately 30-m 3 Teflon reaction bag was designed and built to provide exposures that mimic the production and photochemical oxidation of atmospheric pollutants resulting from the combustion of coal or wood from a stove. The combustion products are introduced into the Teflon bag under atmospheric conditions. Photochemical oxidation of this mixture is accomplished by exposure to tropospheric sun-like radiation from an array of ultraviolet and black lamps. The aerosol in the Teflon reaction bag is then transferred into the exposure room to maintain a constant, lower exposure level. Continuous and semicontinuous monitoring of the gas and particulate matter (PM) pollution in the exposure room and the reaction bag is accomplished using a suite of instruments. This suite of instruments allows for the measurement of the concentrations of total and nonvolatile PM, nitric oxide, nitrogen dioxide, carbon monoxide, carbon dioxide, and ozone. The concentration of the particles was monitored by an R&P tapered element oscillating microbalance monitor. The chemical composition of the PM and its morphological characterization is accomplished by collecting samples in filter packs and conducting ion chromatography, elemental X-ray fluorescence, and scanning electron microscopy analyses. The concentration and composition of emissions from combustion of wood and coal is described. The results of this study suggest that although the bulk compositions of particulate emissions from the combustion of coal or wood in a stove have many similarities, the wood smoke aerosol is photochemically reactive, whereas the coal smoke aerosol is not. IMPLICATIONS The presence of high concentrations of PM and ozone in air has been linked to exacerbation of cardiovascular and cerebrovascular health problems. This manuscript describes the design of a human subject exposure chamber that allows for the production and control of PM and atmospherically important gases and characterizes pollutants present in the exposure chamber from the combustion of wood and coal. Fresh and photochemically aged pollutants are characterized. The chamber thus allows for studies to be conducted to quantify the effects of short-term PM and gas exposure on human subjects.