The ImmunoCAP ISAC molecular allergology approach in adult multi-sensitized Italian patients with respiratory symptoms

To evaluate the performances of an allergen microarray in multi-sensitized allergic patients with respiratory symptoms. 321 patients and 92 controls were included in this study. Specific serum IgE were assayed using ImmunoCAP ISAC, a microarray containing 103 components derived from 47 allergens and...

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Bibliographic Details
Published in:Clinical biochemistry 2011-08, Vol.44 (12), p.1005-1011
Main Authors: Melioli, Giovanni, Bonifazi, Floriano, Bonini, Sergio, Maggi, Enrico, Mussap, Michele, Passalacqua, Gianni, Rossi, Enzo Renato, Vacca, Angelo, Canonica, Giorgio Walter
Format: Article
Language:English
Subjects:
Adolescent
Adult
Aged
Aged, 80 and over
Allergens
Allergens - analysis
Allergens - immunology
Allergic diseases
Allergological tests
Biological and medical sciences
Case-Control Studies
Component resolved diagnostics
Cross Reactions
Female
Humans
Hypersensitivity - complications
Hypersensitivity - diagnosis
Immunoglobulin E - blood
Immunological methods for diagnosis and exploration
Immunopathology
Male
Medical sciences
Microarray
Middle Aged
Molecular allergology
Protein Array Analysis - methods
Reproducibility of Results
Respiratory and ent allergic diseases
Respiratory Tract Diseases - etiology
sIgE
Skin Tests - methods
Young Adult
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Summary:To evaluate the performances of an allergen microarray in multi-sensitized allergic patients with respiratory symptoms. 321 patients and 92 controls were included in this study. Specific serum IgE were assayed using ImmunoCAP ISAC, a microarray containing 103 components derived from 47 allergens and results were compared with extract-based ImmunoCAP Allergens sIgE to 15 common airborne allergens. The reproducibility of ISAC was good. The Positive Percent Agreement (PPA) varied between 75% and 100% for sIgE levels above 1 kUA/l. For samples with sIgE levels below 0.1 kUA/l, the Negative Percent Agreement (NPA) ranged between 90% and 100%. Notably, 58% of respiratory allergy patients had IgE to food-specific proteins and 52% resulted sensitized to cross-reactive pan-allergens. ImmunoCAP ISAC detects allergen sensitization at component level and adds important information by defining both cross and co-sensitization to a large variety of allergen molecules.
ISSN:0009-9120
1873-2933
DOI:10.1016/j.clinbiochem.2011.05.007