Simple Determination of 40 Organophosphate Pesticides in Raw Wool Using Microwave-Assisted Extraction and GC-FPD Analysis

• Published in: Journal of agricultural and food chemistry 2011-07, Vol.59 (14), p.7601-7608
• Main Authors: Niell, Silvina, Pareja, Lucía, González, Gabriel, González, Joaquín, Vryzas, Zisis, Cesio, M. Verónica, Papadopoulou-Mourkidou, Euphemia, Heinzen, Horacio
• Format: Article
• Language: English
• Subjects: Animals; Biological and medical sciences; Chemical Fractionation - instrumentation; Chemical Fractionation - methods; Chromatography, Gas - instrumentation; Chromatography, Gas - methods; detection limit; Food engineering; Food industries; Fundamental and applied biological sciences. Psychology; General aspects; guidelines; Microwaves; multiresidue analysis; Organophosphorus Compounds - analysis; Organophosphorus Compounds - isolation & purification; organophosphorus pesticides; Pesticides - analysis; Pesticides - isolation & purification; Sheep; wool; Wool - chemistry
• ISSN: 0021-8561; 1520-5118
• DOI: 10.1021/jf103983m

A validated analytical method for the multiresidue analysis of 40 organophosphate pesticides (OPs) and conversion products in raw wool has been developed. The method is based on the selective microwave-assisted extraction (MAE) of raw wool with acetonitrile and analysis of extracts by gas chromatography−flame photometric detector. The optimum MAE conditions were 20 min duration at 80 °C with 30 mL of acetonitrile per gram of wool. A validation study was performed according to the European SANCO guidelines 10684/2009. Limits of detection and quantification for all pesticides tested were from 0.01 to 0.2 mg/kg and from 0.2 to 1.0 mg/kg, respectively. The average recoveries of pesticides spiked at different levels were in the range of 70−120% with relative standard deviations of ≤20%. The extraction performance was compared to the one obtained with a reference Soxhlet extraction. The method was also applied in the analysis of real wool (after field application) samples.