Comparative analysis of gelatin scaffolds crosslinked by genipin and silane coupling agent

Scaffolds based on gelatin (G) are considered promising for tissue engineering, able to mimic the natural extracellular matrix. G drawback is its poor structural consistency in wet conditions. Therefore, crosslinking is necessary to fabricate stable G scaffolds. In this work, a comparative study bet...

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Bibliographic Details
Published in:International journal of biological macromolecules 2011-11, Vol.49 (4), p.700-706
Main Authors: Tonda-Turo, C., Gentile, P., Saracino, S., Chiono, V., Nandagiri, V.K., Muzio, G., Canuto, R.A., Ciardelli, G.
Format: Article
Language:English
Subjects:
Animals
Calorimetry, Differential Scanning
Cell Count
Cell Line
Cross-Linking Reagents - chemistry
Elastic Modulus
Gelatin
Gelatin - chemistry
Gelatin - ultrastructure
Genipin
Humans
Iridoid Glycosides - chemistry
Iridoids
Materials Testing
Porosity
Silanes - chemistry
Solubility
Spectroscopy, Fourier Transform Infrared
Sus scrofa
Tissue engineering
Tissue Scaffolds - chemistry
Wettability
γ-Glycidoxypropyltrimethoxysilane
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Summary:Scaffolds based on gelatin (G) are considered promising for tissue engineering, able to mimic the natural extracellular matrix. G drawback is its poor structural consistency in wet conditions. Therefore, crosslinking is necessary to fabricate stable G scaffolds. In this work, a comparative study between the performance of two different crosslinkers, genipin (GP) and γ-glycidoxypropyltrimethoxysilane (GPTMS), is presented. Flat membranes by solvent casting and porous crosslinked scaffolds by freeze-drying were prepared. Infrared spectroscopy and thermal analysis were applied to confirm G chain crosslinking. Moreover, GP and GPTMS increased the stability of G in aqueous media and improved the mechanical properties. Crosslinking reduced the wettability, especially in the case of G_GPTMS samples, due to the introduction of hydrophobic siloxane chains. Both G_GP and G_GPTMS scaffolds supported MG-63 osteoblast-like cell adhesion and proliferation.
ISSN:0141-8130
1879-0003
DOI:10.1016/j.ijbiomac.2011.07.002