Antitussive and immunomodulating activities of instant coffee arabinogalactan-protein

• Published in: International journal of biological macromolecules 2011-11, Vol.49 (4), p.493-497
• Main Authors: Nosáľová, G., Prisenžňáková, L., Paulovičová, E., Capek, P., Matulová, M., Navarini, L., Liverani, F. Suggi
• Format: Article
• Language: English
• Subjects: Administration, Oral; Airway Resistance - drug effects; Animals; Antitussive activity; Antitussive Agents - administration & dosage; Antitussive Agents - pharmacology; Antitussive Agents - therapeutic use; Arabinogalactan-protein; Arabinose; Beans; beta-glucans; cell-mediated immunity; Coffea arabica; Coffee; Coffee - chemistry; Cough - drug therapy; Cough - physiopathology; Galactose; Guinea Pigs; Immune system; Immunity (cell-mediated); Immunologic Factors - administration & dosage; Immunologic Factors - pharmacology; Immunologic Factors - therapeutic use; immunologic techniques; Immunomodulating activity; Inflammation; Instant coffee; interferon-gamma; Interleukin 2; Interleukin-2 - secretion; lymphocytes; Macromolecules; Mice; molecular weight; Mucoproteins - administration & dosage; Mucoproteins - pharmacology; Mucoproteins - therapeutic use; Plant Proteins - administration & dosage; Plant Proteins - pharmacology; Plant Proteins - therapeutic use; Polarization; Powder; Powders; protein content; Spleen - drug effects; Spleen - secretion; tumor necrosis factor-alpha; Tumor Necrosis Factor-alpha - secretion
• ISSN: 0141-8130; 1879-0003
• DOI: 10.1016/j.ijbiomac.2011.06.004

A low molecular mass arabinogalactan-protein (AGP) composed of galactose and arabinose with a low protein content, isolated from the instant coffee powder of Coffea arabica beans, has been tested on antitussive (in vivo) and immunomodulating (ex vivo) activities. The results of antitussive tests revealed a significant dose dependant cough-suppressive effect of coffee AGP. It was observed 30 or 60min after AGP administration and its efficacy lasted during the entire experiment course. Immunological tests showed that AGP affected some mediators of immunocompetent cells of immune system as TNF-α, IFN-γ and IL-2 cytokines. It seems that coffee AGP is a good inductor of both pro-inflammatory cytokines TNF-α and IFN-γ, however, less potent in TNF-α induction in comparison with that of β-d-glucan. Evident induction of TNF-α, IL-2 and IFN-γ cytokines, pro-TH1 polarization supports our conclusion about bio-immunological efficacy of AGP with an emphasis on the cellular immunity.