Supravital fluorometric apoptosis detection in a single mouse embryo using lab-on-a-chip

Detection of apoptosis is one of the main criteria of preimplantation embryo growth potential assessment. Recent developments in lab-on-a-chip techniques has led to apoptosis detection and monitoring on a single cell or embryo level. However, single embryo apoptosis detection without a change in emb...

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Bibliographic Details
Published in:Lab on a chip 2011-10, Vol.11 (19), p.3263-3268
Main Authors: Walczak, Rafał, Śniadek, Patrycja, Dziuban, Jan A, Kluger, Joanna, Soyta, Anna Chełmońska
Format: Article
Language:English
Subjects:
Animals
Apoptosis
Embryo, Mammalian - cytology
Fluorometry - methods
Lab-On-A-Chip Devices
Mice
Miniaturization
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Summary:Detection of apoptosis is one of the main criteria of preimplantation embryo growth potential assessment. Recent developments in lab-on-a-chip techniques has led to apoptosis detection and monitoring on a single cell or embryo level. However, single embryo apoptosis detection without a change in embryo developmental competence and post-examination "recovery" still remains a challenge. In this paper we present a lab-on-a-chip, co-working with miniaturized optical instrumentation, which allows supravital examination of single embryos for the presence of apoptotic blastomers with full after lab-on-a-chip study "recovery" and maintenance of their further developmental capacity.
ISSN:1473-0197
1473-0189
DOI:10.1039/c0lc00667j