Separation of platelets from whole blood using standing surface acoustic waves in a microchannel

Platelet separation from blood is essential for biochemical analyses and clinical diagnosis. In this article, we propose a method to separate platelets from undiluted whole blood using standing surface acoustic waves (SSAWs) in a microfluidic device. A polydimethylsiloxane (PDMS) microfluidic channe...

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Bibliographic Details
Published in:Lab on a chip 2011-10, Vol.11 (19), p.3361-3364
Main Authors: Nam, Jeonghun, Lim, Hyunjung, Kim, Dookon, Shin, Sehyun
Format: Article
Language:English
Subjects:
Acoustics - instrumentation
Blood Platelets - physiology
Cell Separation - instrumentation
Cell Separation - methods
Dimethylpolysiloxanes - chemistry
Flow Cytometry
Humans
Microfluidic Analytical Techniques - instrumentation
Microfluidic Analytical Techniques - methods
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Summary:Platelet separation from blood is essential for biochemical analyses and clinical diagnosis. In this article, we propose a method to separate platelets from undiluted whole blood using standing surface acoustic waves (SSAWs) in a microfluidic device. A polydimethylsiloxane (PDMS) microfluidic channel was fabricated and integrated with interdigitated transducer (IDT) electrodes patterned on a piezoelectric substrate. To avoid shear-induced activation of platelets, the blood sample flow was hydrodynamically focused by introducing sheath flow from two side-inlets and pressure nodes were designed to locate at side walls. By means of flow cytometric analysis, the RBC clearance ratio from whole blood was found to be over 99% and the purity of platelets was close to 98%. Conclusively, the present technique using SSAWs can directly separate platelets from undiluted whole blood with higher purity than other methods.
ISSN:1473-0197
1473-0189
DOI:10.1039/c1lc20346k