Potato Extract (Potein) Suppresses Food Intake in Rats through Inhibition of Luminal Trypsin Activity and Direct Stimulation of Cholecystokinin Secretion from Enteroendocrine Cells

Dietary proteins and trypsin inhibitors are known to stimulate the secretion of the satiety hormone cholecystokinin (CCK). A potato extract (Potein) contains 60% carbohydrate and 20% protein including trypsin inhibitory proteins. In this study, we examined whether Potein suppresses food intake in ra...

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Bibliographic Details
Published in:Journal of agricultural and food chemistry 2011-09, Vol.59 (17), p.9491-9496
Main Authors: Nakajima, Shingo, Hira, Tohru, Tsubata, Masahito, Takagaki, Kinya, Hara, Hiroshi
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Cell Line
cholecystokinin
Cholecystokinin - secretion
dietary protein
Eating - drug effects
Enteroendocrine Cells - drug effects
Enteroendocrine Cells - secretion
Food Chemistry/Biochemistry
food consumption
Food industries
food intake
Fruit and vegetable industries
Fundamental and applied biological sciences. Psychology
Male
oral administration
Plant Extracts - pharmacology
Plant Proteins - pharmacology
potatoes
proteins
Rats
Rats, Sprague-Dawley
Satiation - drug effects
secretion
Solanum tuberosum - chemistry
trypsin
trypsin inhibitors
Trypsin Inhibitors - pharmacology
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Summary:Dietary proteins and trypsin inhibitors are known to stimulate the secretion of the satiety hormone cholecystokinin (CCK). A potato extract (Potein) contains 60% carbohydrate and 20% protein including trypsin inhibitory proteins. In this study, we examined whether Potein suppresses food intake in rats and whether it directly stimulates CCK secretion in enteroendocrine cells. In fasted rats, food consumption was measured up to 6 h after the oral administration of Potein or soybean trypsin inhibitor (SBTI). CCK-releasing activities of Potein and SBTI were examined in the murine CCK-producing cell line STC-1. Potein inhibited the trypsin activity in vitro with a potency 20-fold lower than that of SBTI. Oral administration of Potein dose-dependently suppressed food intake for 1–6 h. Potein, but not the SBTI, dose-dependently induced CCK secretion in STC-1 cells. These results suggest that Potein suppresses food intake through the CCK secretion induced by direct stimulation on enteroendocrine cells and through inhibition of luminal trypsin.
ISSN:0021-8561
1520-5118
DOI:10.1021/jf200988f