Characterization of [6]-gingerol metabolism in rat by liquid chromatography electrospray tandem mass spectrometry

ABSTRACT [6]‐Gingerol is a structural analog of capsaicin, an agonist of the transient receptor potential channel vanilloid 1, which is known to have therapeutic properties for the treatment of pain and inflammation. A selective and sensitive quantitative method for the determination of [6]‐gingerol...

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Bibliographic Details
Published in:Biomedical chromatography 2011-10, Vol.25 (10), p.1150-1158
Main Authors: Gauthier, Marie-Lou, Douat, Jennifer, Vachon, Pascal, Beaudry, Francis
Format: Article
Language:English
Subjects:
Animals
bioanalysis
Calibration
Capsaicin - analysis
Capsaicin - chemistry
Capsaicin - pharmacokinetics
capsaicinoids analog
Catechols - analysis
Catechols - chemistry
Catechols - pharmacokinetics
Chromatography, High Pressure Liquid - methods
Dogs
Fatty Alcohols - analysis
Fatty Alcohols - chemistry
Fatty Alcohols - pharmacokinetics
gingerol
high performance liquid chromatography
Humans
Kinetics
Male
mass spectrometry
metabolism
microsomes
Microsomes, Liver - metabolism
Nonlinear Dynamics
Rats
Rats, Sprague-Dawley
Sensitivity and Specificity
Tandem Mass Spectrometry - methods
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Summary:ABSTRACT [6]‐Gingerol is a structural analog of capsaicin, an agonist of the transient receptor potential channel vanilloid 1, which is known to have therapeutic properties for the treatment of pain and inflammation. A selective and sensitive quantitative method for the determination of [6]‐gingerol by HPLC‐ESI/MS/MS was developed. The method consisted of a protein precipitation extraction followed by analysis using liquid chromatography electrospray tandem mass spectrometry. The chromatographic separation was achieved using a Thermo 100 × 2.1 mm C8 column combined with an isocratic mobile phase composed of acetonitrile, water and formic acid (80:20:0.1) at a flow rate of 250 μL/min. The mass spectrometer was operating in SRM mode and an analytical range set at 20–5000 ng/mL was used to construct a calibration curve in rat plasma. The interbatch precision (%CV) and accuracy (%NOM) observed were 2.9–10.8% and 98.1–102.1% in rat plasma. Similarly, precision and accuracy in rat liver microsomal suspension were also evaluated at nominal concentrations of 1, 25 and 100 μm; the precision (%CV) was
ISSN:0269-3879
1099-0801
DOI:10.1002/bmc.1585