Molecular Genetic Study of Species and Strain Variability in Bifidobacteria Population in Intestinal Microflora of Breast-Fed Infants and Their Mothers

Qualitative and quantitative composition of enteric bifidoflora was studied in a group of 13 mother-infant pairs. Pure cultures of Bifidobacterium strains were isolated from feces and their species were identified by PCR with species-specific primers or by partial sequencing of 16S rDNA. The strains...

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Bibliographic Details
Published in:Bulletin of experimental biology and medicine 2010-12, Vol.150 (1), p.61-64
Main Authors: Kulagina, E. V, Shkoporov, A. N, Kafarskaia, L. I, Khokhlova, E. V, Volodin, N. N, Donskikh, E. E, Korshunova, O. V, Efimov, B. A
Format: Article
Language:English
Subjects:
Adult
bifidobacteria
Bifidobacterium
Bifidobacterium - classification
Bifidobacterium - genetics
Biomedical and Life Sciences
Biomedicine
Breast Feeding
Cell Biology
enteric microflora
Feces - microbiology
Female
Food and nutrition
Genetic research
Humans
Infant
Infants
Internal Medicine
Intestines - microbiology
Laboratory Medicine
Pathology
Polymerase Chain Reaction
REP-PCR
Young Adult
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Summary:Qualitative and quantitative composition of enteric bifidoflora was studied in a group of 13 mother-infant pairs. Pure cultures of Bifidobacterium strains were isolated from feces and their species were identified by PCR with species-specific primers or by partial sequencing of 16S rDNA. The strains were compared by REP-PCR. The most incident Bifidobacterium species in mothers were B. longum and B. adolescentis. The infants were mainly colonized by B. bifdum and B. longum. The mother and her baby were colonized by the same Bifidobacterium species in 9 of 13 cases. In 5 (38.5%) of these cases, these pairs of strains were identical by their REP-PCR profiles. These strains belonged to B. longum in one case, B. bifidum in 3 cases, and B. adolescentis in 1 case. Our results support the hypothesis on early colonization of infants with maternal bifidobacterium strains.
ISSN:0007-4888
1573-8221
DOI:10.1007/s10517-010-1069-y