Gluconacetobacter diazotrophicus levansucrase is involved in tolerance to NaCl, sucrose and desiccation, and in biofilm formation

• Published in: Archives of microbiology 2011-02, Vol.193 (2), p.137-149
• Main Authors: Velázquez-Hernández, M. Lourdes, Baizabal-Aguirre, Víctor M., Cruz-Vázquez, Fermín, Trejo-Contreras, Mayra J., Fuentes-Ramírez, Luis E., Bravo-Patiño, Alejandro, Cajero-Juárez, Marcos, Chávez-Moctezuma, Martha P., Valdez-Alarcón, Juan J.
• Format: Article
• Language: English
• Subjects: Aggregates; Bacteria; Bacteriology; Biochemistry; Biofilms; Biofilms - drug effects; Biofilms - growth & development; Biological and medical sciences; Biomedical and Life Sciences; Biotechnology; Biotic factors; Cell Biology; Desiccation; Dilution; Ecology; Fructans - biosynthesis; Fundamental and applied biological sciences. Psychology; Genes; Genetic Complementation Test; Gluconacetobacter - enzymology; Gluconacetobacter - genetics; Gluconacetobacter - physiology; Gluconacetobacter diazotrophicus; Glucose; Glycerol; Hexosyltransferases - genetics; Hexosyltransferases - metabolism; Hypertonic Solutions - pharmacology; Life Sciences; Microbial Ecology; Microbiology; Miscellaneous; Mutation; Nitrogen; Nitrogen fixation; Original Paper; Physical properties; Plasmids; Polyethylene Glycols - pharmacology; Sodium chloride; Sodium Chloride - pharmacology; Sucrose; Sucrose - pharmacology; Sugarcane
• ISSN: 0302-8933; 1432-072X
• DOI: 10.1007/s00203-010-0651-z

Gluconacetobacter diazotrophicus is a nitrogen-fixing bacterium and endophyte of sugarcane, which expresses levansucrase, a fructosyltransferase exoenzyme with sucrose hydrolytic and levan biosynthetic activities. As a result of their physical properties, the levan can provide protection against stress caused by abiotic or biotic factors and participate in the formation of biofilms. In this study, we investigated the construction and function of a levansucrase-defective mutant of G. diazotrophicus . The lsdA mutant showed a decreased tolerance (65.5%) to 50–150 mM NaCl and a decrease of 89% in 876 mM (30%) sucrose, a reduction (99%) in tolerance to desiccation after 18 h, and a decrease (36.9–58.5%) in the ability to form cell aggregates on abiotic surfaces. Complementation of the mutant with the complete lsdA gene leads to a recovery of the ability to grow on sucrose-containing medium and to form slimy colonies, the ability to form the cell aggregates on abiotic surfaces and the tolerance to NaCl. This report demonstrates the importance of levansucrase in environmental adaptation of G. diazotrophicus under high osmotic stress and in biofilm formation.