Development and characterization of three new diploid cell lines from Labeo rohita (Ham.)

Development of cell lines from fish for identifying the pathogenesis of viral diseases and for vaccine production against viral and bacterial diseases is imperative where they are of commercial importance. Three new diploid fish cell lines (RF, RH, and RSB) were developed from fin, heart, and swim b...

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Bibliographic Details
Published in:Biotechnology progress 2010-07, Vol.26 (4), p.1008-1013
Main Authors: Lakra, Wazir S., Swaminathan, T. Raja, Rathore, Gaurav, Goswami, Mukunda, Yadav, Kamalendra, Kapoor, Smita
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Biotechnology
Carps
Cell Culture Techniques - methods
Cell Line
characterization
Chromosomes
Cytochrome-c oxidase
development
Diploids
Diploidy
Fundamental and applied biological sciences. Psychology
genomics
Ham
Heart
Labeo rohita
Mitochondrial DNA
Nitrogen
rRNA 16S
Swim bladder
Vaccines
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Summary:Development of cell lines from fish for identifying the pathogenesis of viral diseases and for vaccine production against viral and bacterial diseases is imperative where they are of commercial importance. Three new diploid fish cell lines (RF, RH, and RSB) were developed from fin, heart, and swim bladder of an Indian major carp, Labeo rohita, commonly called Rohu. All the cell lines were optimally maintained at 28°C in Leibovitz‐15 medium supplemented with 10% FBS. The propagation of RH and RSB cells was serum dependent, with a low plating efficiency (
ISSN:8756-7938
1520-6033
1520-6033
DOI:10.1002/btpr.418