A high-capacity, hybrid electro-microneedle for in-situ cutaneous gene transfer

Abstract Cutaneous gene transfer is limited by biological barriers such as skin and cellular membranes; complex approaches are required to overcome these biological barriers, simultaneously. Non-integrated systems that separate cutaneous permeation from intracellular transfection have been used to o...

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Bibliographic Details
Published in:Biomaterials 2011-10, Vol.32 (30), p.7705-7710
Main Authors: Lee, Kwang, Kim, Jung Dong, Lee, Chang Yoel, Her, Song, Jung, Hyungil
Format: Article
Language:English
Subjects:
Administration, Cutaneous
Advanced Basic Science
Animals
Cell Line, Tumor
Cutaneous gene transfer
Dentistry
DNA - administration & dosage
DNA - genetics
Drawing lithography
Equipment Design
Gene Transfer Techniques - instrumentation
Hybrid electro-microneedle
Integrated system
Interleukin-12 - genetics
Melanoma - genetics
Melanoma - therapy
Mice
Mice, Hairless
Mice, Inbred C57BL
Needles
Skin - metabolism
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Summary:Abstract Cutaneous gene transfer is limited by biological barriers such as skin and cellular membranes; complex approaches are required to overcome these biological barriers, simultaneously. Non-integrated systems that separate cutaneous permeation from intracellular transfection have been used to overcome skin and cellular barriers, respectively, however, do not provide sufficient doses of the gene to local tissue, resulting in inefficient gene transfer in-situ . Although integrated systems for cutaneous gene transfer are available, their safety has been questioned and it is difficult to transfer sufficient amounts of genes due to cumbersome sterilization procedures and the small size of the reservoir. Here, we demonstrate stepwise-aligned cutaneous permeation, cutaneous release, and intracellular transfection using a hybrid electro-microneedle (HEM), which designed as a monolithic hybrid assembly of a dissolving microneedle and an electrode, anomalously. Furthermore, as proof-of-principle, we use the HEM for in-situ cutaneous transfer of p2CMVmIL-12 to successfully treat B16F10 subcutaneous tumors in a mouse model. The HEM described herein holds great promise for cutaneous gene therapy of cancers and for vaccines.
ISSN:0142-9612
1878-5905
DOI:10.1016/j.biomaterials.2011.06.058