Control of the activity of the soluble lytic transglycosylase by the stringent response in Escherichia coli

The soluble lytic transglycosylase (Slt) of Escherichia coli is known to be a powerful murein hydrolase in vitro. It is shown here to act as an autolysin in vivo as well. Rapid autolysis of Slt overproducing cells was induced by protein bio‐synthesis inhibitors, which also block the fomration of gua...

Full description

Saved in:
Bibliographic Details
Published in:FEMS microbiology letters 1990-01, Vol.67 (1‐2), p.161-164
Main Authors: Betzner, A.S., Ferreira, L.C.S., Höltje, J.‐V., Keck, W.
Format: Article
Language:English
Subjects:
Autolysis
Bacteriology
Biological and medical sciences
Escherichia coli
Fundamental and applied biological sciences. Psychology
Metabolism. Enzymes
Microbiology
Murein
RelA
Salt
Soluble lytic transglycosylase
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
cited_by cdi_FETCH-LOGICAL-c3421-888e0178e2b4f932631230dfa39c8e1deef5b59525970763899b2225710dfe9e3
cites cdi_FETCH-LOGICAL-c3421-888e0178e2b4f932631230dfa39c8e1deef5b59525970763899b2225710dfe9e3
container_end_page 164
container_issue 1‐2
container_start_page 161
container_title FEMS microbiology letters
container_volume 67
creator Betzner, A.S.
Ferreira, L.C.S.
Höltje, J.‐V.
Keck, W.
description The soluble lytic transglycosylase (Slt) of Escherichia coli is known to be a powerful murein hydrolase in vitro. It is shown here to act as an autolysin in vivo as well. Rapid autolysis of Slt overproducing cells was induced by protein bio‐synthesis inhibitors, which also block the fomration of guanosine‐5′‐diphosphate‐3′‐diphosphate (ppGpp). When amino acid starvation was used to in relA+ but not in relA− cells. These findings indicate that the stringent control modulates the enzymatic activity of the soluble lytic transglycosylase in vivo.
doi_str_mv 10.1111/j.1574-6968.1990.tb13855.x
format article
fullrecord <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_888104165</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>888104165</sourcerecordid><originalsourceid>FETCH-LOGICAL-c3421-888e0178e2b4f932631230dfa39c8e1deef5b59525970763899b2225710dfe9e3</originalsourceid><addsrcrecordid>eNqVkE2LFDEQhoMoOK7-hyCCp25TSSedeBBk2A9hxIt7Duls9U7GbGdMMrr977eHGfduXYqinqoXHkLeA2thqU-7FmTfNcoo3YIxrK0DCC1l-_iCrJ5XL8mKiV43wEz_mrwpZccY6zhTK_JrnaaaU6RppHWL1Pka_oQ6_5tLiochIo1zDZ7W7KZyH2efyhxdQTrMJ6rmMN3jVGnGsk_TsgkTvSx-izn4bXDUpxjeklejiwXfnfsFub26_Lm-aTY_rr-tv24aLzoOjdYaGfQa-dCNRnAlgAt2NzphvEa4QxzlII3k0vSsV0IbM3DOZQ8LhAbFBfl4-rvP6fcBS7UPoXiM0U2YDsUuAcA6UHIhP59In1MpGUe7z-HB5dkCs0fBdmePFu3Roj0KtmfB9nE5_nCOccW7OC5ufCjPH5TuGYBasC8n7G-IOP9HgL36vgEF4gkqiZD3</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>888104165</pqid></control><display><type>article</type><title>Control of the activity of the soluble lytic transglycosylase by the stringent response in Escherichia coli</title><source>Alma/SFX Local Collection</source><creator>Betzner, A.S. ; Ferreira, L.C.S. ; Höltje, J.‐V. ; Keck, W.</creator><creatorcontrib>Betzner, A.S. ; Ferreira, L.C.S. ; Höltje, J.‐V. ; Keck, W.</creatorcontrib><description>The soluble lytic transglycosylase (Slt) of Escherichia coli is known to be a powerful murein hydrolase in vitro. It is shown here to act as an autolysin in vivo as well. Rapid autolysis of Slt overproducing cells was induced by protein bio‐synthesis inhibitors, which also block the fomration of guanosine‐5′‐diphosphate‐3′‐diphosphate (ppGpp). When amino acid starvation was used to in relA+ but not in relA− cells. These findings indicate that the stringent control modulates the enzymatic activity of the soluble lytic transglycosylase in vivo.</description><identifier>ISSN: 0378-1097</identifier><identifier>EISSN: 1574-6968</identifier><identifier>DOI: 10.1111/j.1574-6968.1990.tb13855.x</identifier><identifier>CODEN: FMLED7</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>Autolysis ; Bacteriology ; Biological and medical sciences ; Escherichia coli ; Fundamental and applied biological sciences. Psychology ; Metabolism. Enzymes ; Microbiology ; Murein ; RelA ; Salt ; Soluble lytic transglycosylase</subject><ispartof>FEMS microbiology letters, 1990-01, Vol.67 (1‐2), p.161-164</ispartof><rights>1990 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3421-888e0178e2b4f932631230dfa39c8e1deef5b59525970763899b2225710dfe9e3</citedby><cites>FETCH-LOGICAL-c3421-888e0178e2b4f932631230dfa39c8e1deef5b59525970763899b2225710dfe9e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,4010,27900,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&amp;idt=6870116$$DView record in Pascal Francis$$Hfree_for_read</backlink></links><search><creatorcontrib>Betzner, A.S.</creatorcontrib><creatorcontrib>Ferreira, L.C.S.</creatorcontrib><creatorcontrib>Höltje, J.‐V.</creatorcontrib><creatorcontrib>Keck, W.</creatorcontrib><title>Control of the activity of the soluble lytic transglycosylase by the stringent response in Escherichia coli</title><title>FEMS microbiology letters</title><description>The soluble lytic transglycosylase (Slt) of Escherichia coli is known to be a powerful murein hydrolase in vitro. It is shown here to act as an autolysin in vivo as well. Rapid autolysis of Slt overproducing cells was induced by protein bio‐synthesis inhibitors, which also block the fomration of guanosine‐5′‐diphosphate‐3′‐diphosphate (ppGpp). When amino acid starvation was used to in relA+ but not in relA− cells. These findings indicate that the stringent control modulates the enzymatic activity of the soluble lytic transglycosylase in vivo.</description><subject>Autolysis</subject><subject>Bacteriology</subject><subject>Biological and medical sciences</subject><subject>Escherichia coli</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Metabolism. Enzymes</subject><subject>Microbiology</subject><subject>Murein</subject><subject>RelA</subject><subject>Salt</subject><subject>Soluble lytic transglycosylase</subject><issn>0378-1097</issn><issn>1574-6968</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1990</creationdate><recordtype>article</recordtype><recordid>eNqVkE2LFDEQhoMoOK7-hyCCp25TSSedeBBk2A9hxIt7Duls9U7GbGdMMrr977eHGfduXYqinqoXHkLeA2thqU-7FmTfNcoo3YIxrK0DCC1l-_iCrJ5XL8mKiV43wEz_mrwpZccY6zhTK_JrnaaaU6RppHWL1Pka_oQ6_5tLiochIo1zDZ7W7KZyH2efyhxdQTrMJ6rmMN3jVGnGsk_TsgkTvSx-izn4bXDUpxjeklejiwXfnfsFub26_Lm-aTY_rr-tv24aLzoOjdYaGfQa-dCNRnAlgAt2NzphvEa4QxzlII3k0vSsV0IbM3DOZQ8LhAbFBfl4-rvP6fcBS7UPoXiM0U2YDsUuAcA6UHIhP59In1MpGUe7z-HB5dkCs0fBdmePFu3Roj0KtmfB9nE5_nCOccW7OC5ufCjPH5TuGYBasC8n7G-IOP9HgL36vgEF4gkqiZD3</recordid><startdate>199001</startdate><enddate>199001</enddate><creator>Betzner, A.S.</creator><creator>Ferreira, L.C.S.</creator><creator>Höltje, J.‐V.</creator><creator>Keck, W.</creator><general>Blackwell Publishing Ltd</general><general>Blackwell</general><scope>IQODW</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>C1K</scope></search><sort><creationdate>199001</creationdate><title>Control of the activity of the soluble lytic transglycosylase by the stringent response in Escherichia coli</title><author>Betzner, A.S. ; Ferreira, L.C.S. ; Höltje, J.‐V. ; Keck, W.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3421-888e0178e2b4f932631230dfa39c8e1deef5b59525970763899b2225710dfe9e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1990</creationdate><topic>Autolysis</topic><topic>Bacteriology</topic><topic>Biological and medical sciences</topic><topic>Escherichia coli</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Metabolism. Enzymes</topic><topic>Microbiology</topic><topic>Murein</topic><topic>RelA</topic><topic>Salt</topic><topic>Soluble lytic transglycosylase</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Betzner, A.S.</creatorcontrib><creatorcontrib>Ferreira, L.C.S.</creatorcontrib><creatorcontrib>Höltje, J.‐V.</creatorcontrib><creatorcontrib>Keck, W.</creatorcontrib><collection>Pascal-Francis</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Environmental Sciences and Pollution Management</collection><jtitle>FEMS microbiology letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Betzner, A.S.</au><au>Ferreira, L.C.S.</au><au>Höltje, J.‐V.</au><au>Keck, W.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Control of the activity of the soluble lytic transglycosylase by the stringent response in Escherichia coli</atitle><jtitle>FEMS microbiology letters</jtitle><date>1990-01</date><risdate>1990</risdate><volume>67</volume><issue>1‐2</issue><spage>161</spage><epage>164</epage><pages>161-164</pages><issn>0378-1097</issn><eissn>1574-6968</eissn><coden>FMLED7</coden><abstract>The soluble lytic transglycosylase (Slt) of Escherichia coli is known to be a powerful murein hydrolase in vitro. It is shown here to act as an autolysin in vivo as well. Rapid autolysis of Slt overproducing cells was induced by protein bio‐synthesis inhibitors, which also block the fomration of guanosine‐5′‐diphosphate‐3′‐diphosphate (ppGpp). When amino acid starvation was used to in relA+ but not in relA− cells. These findings indicate that the stringent control modulates the enzymatic activity of the soluble lytic transglycosylase in vivo.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><doi>10.1111/j.1574-6968.1990.tb13855.x</doi><tpages>4</tpages><oa>free_for_read</oa></addata></record>
fulltext fulltext
identifier ISSN: 0378-1097
ispartof FEMS microbiology letters, 1990-01, Vol.67 (1‐2), p.161-164
issn 0378-1097
1574-6968
language eng
recordid cdi_proquest_miscellaneous_888104165
source Alma/SFX Local Collection
subjects Autolysis
Bacteriology
Biological and medical sciences
Escherichia coli
Fundamental and applied biological sciences. Psychology
Metabolism. Enzymes
Microbiology
Murein
RelA
Salt
Soluble lytic transglycosylase
title Control of the activity of the soluble lytic transglycosylase by the stringent response in Escherichia coli
url http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-01T15%3A51%3A27IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Control%20of%20the%20activity%20of%20the%20soluble%20lytic%20transglycosylase%20by%20the%20stringent%20response%20in%20Escherichia%20coli&rft.jtitle=FEMS%20microbiology%20letters&rft.au=Betzner,%20A.S.&rft.date=1990-01&rft.volume=67&rft.issue=1%E2%80%902&rft.spage=161&rft.epage=164&rft.pages=161-164&rft.issn=0378-1097&rft.eissn=1574-6968&rft.coden=FMLED7&rft_id=info:doi/10.1111/j.1574-6968.1990.tb13855.x&rft_dat=%3Cproquest_cross%3E888104165%3C/proquest_cross%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c3421-888e0178e2b4f932631230dfa39c8e1deef5b59525970763899b2225710dfe9e3%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=888104165&rft_id=info:pmid/&rfr_iscdi=true