Stimulation of glucose oxidase with white linearly polarized light

Glucose oxidase (GOD) was illuminated with white linearly polarized light (WLPL). The enzyme was illuminated at room temperature in separate vessels then admixed to a reactor filled with D‐glucose. The illumination of the enzyme for 60 min at 25–30°C and pH 6.5–7.0 provided its superior stimulation...

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Bibliographic Details
Published in:Biotechnology progress 2010-03, Vol.26 (2), p.393-396
Main Authors: Konieczna-Molenda, Anna, Fiedorowicz, Maciej, Tomasik, Przemysław J.
Format: Article
Language:English
Subjects:
Biological and medical sciences
Biotechnology
enzymatic hydrolysis
Freeze Drying
Fundamental and applied biological sciences. Psychology
Glucose - metabolism
glucose oxidase
Glucose Oxidase - chemistry
Glucose Oxidase - metabolism
Glucose Oxidase - radiation effects
Hydrogen-Ion Concentration
Hydrolysis
Light
polarized light
Temperature
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Summary:Glucose oxidase (GOD) was illuminated with white linearly polarized light (WLPL). The enzyme was illuminated at room temperature in separate vessels then admixed to a reactor filled with D‐glucose. The illumination of the enzyme for 60 min at 25–30°C and pH 6.5–7.0 provided its superior stimulation as proven in the oxidation of β‐D‐glucose. Lyophilization of the illuminated enzyme reduced its activity by, approximately, 30%. © 2009 American Institute of Chemical Engineers Biotechnol. Prog., 2010
ISSN:8756-7938
1520-6033
1520-6033
DOI:10.1002/btpr.330