Lineage restricted progenitors for the repopulation of decellularized heart

Abstract The severe shortage of available donor hearts necessitates the development of other options for heart replacement. Recent results underline the promise of the decellularized organ approach in engineering a functional heart. However, little is known so far regarding the ability of decellular...

Full description

Saved in:
Bibliographic Details
Published in:Biomaterials 2011-10, Vol.32 (30), p.7571-7580
Main Authors: Ng, Serina L.J, Narayanan, Karthikeyan, Gao, Shujun, Wan, Andrew C.A
Format: Article
Language:English
Subjects:
Advanced Basic Science
Animals
Cell Differentiation
Cell Line
Dentistry
ECM (extracellular matrix)
Embryonic Stem Cells - cytology
Embryonic Stem Cells - metabolism
Embryonic Stem Cells - transplantation
Extracellular Matrix - metabolism
Heart
Heart - physiology
Humans
Mice
Mice, SCID
Myocardium - cytology
Progenitor cell
Stem cell
Stem Cell Transplantation
Stem Cells - cytology
Stem Cells - metabolism
Tissue Engineering - methods
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Abstract The severe shortage of available donor hearts necessitates the development of other options for heart replacement. Recent results underline the promise of the decellularized organ approach in engineering a functional heart. However, little is known so far regarding the ability of decellularized heart ECM to differentiate embryonic stem cells or committed progenitor cells. In the present work, we compared the differentiation potential of human embryonic stem cells (hESCs) and human mesendodermal cells (hMECs) derived from hESCs, in decellularized hearts under static culture. Expression of various cardiac specific markers such as cTnT, Nkx-2.5, Myl2, Myl7, Myh6 and CD31 was elucidated by gene expression, immunostaining and flow cytometry. Both hMECs and hESCs upregulated expression of cardiac markers upon differentiation, but they exclusively expressed genes for myosin light chain (Myl2, Myl7) and myosin heavy chain (Myh6), respectively. To enhance the differentiation ability of the stem/progenitor cells in the acellular constructs, they were implanted subcutaneously in SCID mice. Immunostaining of the explants revealed the persistence of cardiac marker expressing cells, but which lacked beating function. Our results indicate that the intact extracellular matrix components and preserved mechanical properties of the decellularized heart had directed differentiation of the stem/progenitor cells into the cardiac lineage.
ISSN:0142-9612
1878-5905
DOI:10.1016/j.biomaterials.2011.06.065