Effect of lignocellulosic inhibitory compounds on growth and ethanol fermentation of newly-isolated thermotolerant Issatchenkia orientalis

• Published in: Bioresource technology 2011-09, Vol.102 (17), p.8099-8104
• Main Authors: KWON, Yong-Jin, MA, An-Zhou, QIAN LI, FENG WANG, ZHUANG, Guo-Qiang, LIU, Chun-Zhao
• Format: Article
• Language: English
• Subjects: Adaptation, Physiological; Base Sequence; Biological and medical sciences; Bioreactors; Biotechnology; DNA Primers; Ethanol; Ethanol - metabolism; Ethyl alcohol; Fermentation; Fundamental and applied biological sciences. Psychology; Furfural; Glucose; Hydrolysis; Issatchenkia orientalis; Lignin - antagonists & inhibitors; Methods. Procedures. Technologies; Microbial engineering. Fermentation and microbial culture technology; Polymerase Chain Reaction; Productivity; Strain; Temperature; Vanillin; Yeasts - drug effects; Yeasts - growth & development; Yeasts - metabolism; Yeasts - physiology
• ISSN: 0960-8524; 1873-2976
• DOI: 10.1016/j.biortech.2011.06.035

A newly isolated thermotolerant ethanologenic yeast strain, Issatchenkia orientalis IPE 100, was able to produce ethanol with a theoretical yield of 85% per g of glucose at 42°C. Ethanol production was inhibited by furfural, hydroxymethylfurfural and vanillin concentrations above 5.56 gL(-1), 7.81 gL(-1), and 3.17 gL(-1), respectively, but the strain was able to produce ethanol from enzymatically hydrolyzed steam-exploded cornstalk with 93.8% of theoretical yield and 0.91 gL(-1)h(-1) of productivity at 42°C. Therefore, I. orientalis IPE 100 is a potential candidate for commercial lignocelluloses-to-ethanol production.