Current status of the metabolic engineering of microorganisms for biohydrogen production

► Microbial biohydrogen production processes: photolysis, photo- and dark-fermentation. ► Metabolic engineering in improving the H 2 production capabilities of microorganisms. ► Significant improvements in terms of activity, yield and metabolic robustness. ► Review of recent developments in H 2 prod...

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Bibliographic Details
Published in:Bioresource technology 2011-09, Vol.102 (18), p.8357-8367
Main Authors: Oh, You-Kwan, Raj, Subramanian Mohan, Jung, Gyoo Yeol, Park, Sunghoon
Format: Article
Language:English
Subjects:
Bacteria - metabolism
Biofuels - analysis
Biohydrogen
Biophotolysis
Carbon
Dark fermentation
Enzymes
Fermentation - physiology
Flux
Hydrogen - metabolism
Metabolic engineering
Metabolic Engineering - trends
Metabolism
Microorganisms
Pathways
Photofermentation
Photolysis
Stability
Utilization
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Summary:► Microbial biohydrogen production processes: photolysis, photo- and dark-fermentation. ► Metabolic engineering in improving the H 2 production capabilities of microorganisms. ► Significant improvements in terms of activity, yield and metabolic robustness. ► Review of recent developments in H 2 production with a focus on metabolic engineering. The improvement of H 2 production capabilities of hydrogen (H 2)-producing microorganisms is a challenging issue. Microorganisms have evolved for fast growth and substrate utilization rather than H 2 production. To develop good H 2-producing biocatalysts, many studies have focused on the redirection and/or reconstruction of cellular metabolisms. These studies included the elimination of enzymes and carbon pathways interfering or competing with H 2 production, the incorporation of non-native metabolic pathways leading to H 2 production, the utilization of various carbon substrates, the rectification of H 2-producting enzymes (nitrogenase and hydrogenase) and photophosphorylation systems, and in silico pathway flux analysis, among others. Owing to these studies, significant improvements in the yield and rate of H 2 production, and in the stability of H 2 production activity, were reached. This review presents and discusses the recent developments in biohydrogen production, with a focus on metabolic pathway engineering.
ISSN:0960-8524
1873-2976
DOI:10.1016/j.biortech.2011.04.054