Isolation and characterization of microsatellite markers for a worldwide invasive weed, Chromolaena odorata (Asteraceae)

• Premise of the study: Microsatellite loci were isolated and characterized for a worldwide invasive weed Chromolaena odorata (Asteraceae) to elucidate the population genetic structure and invasive history. • Methods and Results: A total of 14 microsatellite primer pairs were developed using the Fas...

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Bibliographic Details
Published in:American journal of botany 2011-09, Vol.98 (9), p.e259-e261
Main Authors: Yu, Xiang‐Qin, Li, Qiao‐Ming
Format: Article
Language:English
Subjects:
Asteraceae - genetics
Base Sequence
Botany
Chromolaena odorata
DNA Primers
Flowers & plants
Genes, Plant
Genetic Markers
invasive history
invasive weed
Microsatellite Repeats - genetics
microsatellites
Nonnative species
Polymerase Chain Reaction
Polymorphism
Polymorphism, Genetic
Population genetics
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Summary:• Premise of the study: Microsatellite loci were isolated and characterized for a worldwide invasive weed Chromolaena odorata (Asteraceae) to elucidate the population genetic structure and invasive history. • Methods and Results: A total of 14 microsatellite primer pairs were developed using the Fast Isolation by AFLP of Sequences Containing repeats (FIASCO) protocol, and their polymorphism was assessed in two natural populations of C. odorata from Mexico and Trinidad and Tobago. Eleven loci showed polymorphism and eight of these loci were successfully amplified in Ageratina adenophora, another invasive weed related to C. odorata. • Conclusions: These microsatellite markers are useful for investigating the population genetic structure and the history of range expansion of these invasive species.
ISSN:0002-9122
1537-2197
DOI:10.3732/ajb.1100169