What does it take to resolve relationships and to identify species with molecular markers? An example from the epiphytic Rhipsalideae (Cactaceae)

• Published in: American journal of botany 2011-09, Vol.98 (9), p.1549-1572
• Main Authors: Korotkova, Nadja, Borsch, Thomas, Quandt, Dietmar, Taylor, Nigel P., Müller, Kai F., Barthlott, Wilhelm
• Format: Article
• Language: English
• Subjects: Biological taxonomies; Cactaceae; Cactaceae - genetics; Cactus; Datasets; DNA barcoding; DNA Primers; DNA, Plant - genetics; Flowers; Flowers & plants; Genera; Genes, Plant; Genetic Markers; Hatiora; Introns; inversion; Lepismium; Nonnative species; Phylogenetics; Plants; Plastids; Rhipsalidopsis; Rhipsalis; Schlumbergera; species‐level phylogeny; Systematics and Phytogeography; Taxa; Taxonomy
• ISSN: 0002-9122; 1537-2197
• DOI: 10.3732/ajb.1000502

Premise of the study: The Cactaceae are a major New World plant family and popular in horticulture. Still, taxonomie units and species limits have been difficult to define, and molecular phylogenetic studies so far have yielded largely unresolved trees, so relationships within Cactaceae remain insufficiently understood. This study focuses on the predominantly epiphytic tribe Rhipsalideae and evaluates the utility of a spectrum of plastid genomic regions. Methods: We present a phylogenetic study including 52 of the 53 Rhipsalideae species and all the infraspecific taxa. Seven regions (trnK intron, matK, rbcL, rps3-rpl16, rpl16 intron, psbA-trnH, trnQ-rps16), ca. 5600 nucleotides (nt) were sequenced per sample. The regions used were evaluated for their phylogenetic performance and performance in DNA-based species recognition based on operational taxonomie units (OTUs) defined beforehand. Key results: The Rhipsalideae are monophyletic and contain five clades that correspond to the genera Rhipsalis, Lepismium, Schlumbergera, Hatiora, and Rhipsalidopsis. The species-level tree was well resolved and supported; the rpl16 and trnK introns yielded the best phylogenetic signal. Although the psbA-trnH and trnQ-rps16 spacers were the most successful individual regions for OTU identification, their success rate did not significantly exceed 70%. The highest OTU identification rate of 97% was found using the combination of psbA-trnH, rps3-rpl16, trnK intron, and trnQ-rps16 as a minimum possible marker length (ca. 1660 nt). Conclusions: The phylogenetic performance of a marker is not determined by the level of sequence variability, and species discrimination power does not necessarily correlate with phylogenetic utility.