Quantitative analysis of structural disorder in intervertebral disks using second harmonic generation imaging: comparison with morphometric analysis

A novel signal processing algorithm for quantifying structural disorder in biological tissue using second harmonic generation (SHG) imaging is described. Both the magnitude and the pattern of disorder in collagenous tissues can be determined with this method. Mathematical models are used to determin...

Full description

Saved in:
Bibliographic Details
Published in:Journal of Biomedical Optics 2007-11, Vol.12 (6), p.064019-0640117
Main Authors: Reiser, Karen M, Bratton, Clayton, Yankelevich, Diego R, Knoesen, Andre´, Rocha-Mendoza, Israel, Lotz, Jeffrey
Format: Article
Language:English
Subjects:
Algorithms
Animals
Biological
Collagen - metabolism
collagen structure
disorder
Disorders
Hot Temperature - adverse effects
Image Processing, Computer-Assisted
Imaging
Intervertebral Disc - injuries
Intervertebral Disc - metabolism
Intervertebral Disc - pathology
intervertebral disk
Intervertebral disks
Mathematical models
Mathematics
Mice
Nonlinear Dynamics
Optics and Photonics
Photomicrography
Rats
Scanning
Second harmonic generation
Signal Processing, Computer-Assisted
Tendon Injuries - metabolism
Tendon Injuries - pathology
Tendons - metabolism
Tendons - pathology
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:A novel signal processing algorithm for quantifying structural disorder in biological tissue using second harmonic generation (SHG) imaging is described. Both the magnitude and the pattern of disorder in collagenous tissues can be determined with this method. Mathematical models are used to determine the range of disordered states over which the algorithm can be used, because highly disordered biological samples do not generate second harmonic signals. The method is validated by measuring disorder in heated fascicles using SHG and showing that results are significantly correlated with morphometric determination. Applicability of the method to tissue pathology is demonstrated by analysis of a mouse model of intervertebral disk injury. Disks were subjected to tensile or compressive forces for one week. Structural disorder in the annulus fibrosus was measured by SHG scanning and by standard morphometric analysis. Values for disorder obtained by SHG scanning were significantly correlated with values obtained by morphometry . Quantitation of disorder using SHG offers significant advantages over morphometric determination. Data obtained in this study suggest that this method can be used to discriminate between reversible and irreversible tissue damage.
ISSN:1083-3668
1560-2281
DOI:10.1117/1.2812631