Multifunctional microbubbles for image-guided antivascular endothelial growth factor therapy

We synthesize multifunctional microbubbles (MBs) for targeted delivery of antivascular endothelial growth factor (antiVEGF) therapy with multimodal imaging guidance. Poly-lactic-co-glycolic acid (PLGA) MBs encapsulating Texas Red dye are fabricated by a modified double-emulsion process. Simultaneous...

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Bibliographic Details
Published in:Journal of Biomedical Optics 2010-05, Vol.15 (3), p.030515-030515
Main Authors: Zhang, Leilei, Xu, Jeff S, Sanders, Virgil E, Letson, Alan D, Roberts, Cynthia J, Xu, Ronald X
Format: Article
Language:English
Subjects:
Angiogenesis Inhibitors - administration & dosage
Angiogenesis Inhibitors - chemistry
Antibodies, Monoclonal - administration & dosage
Antibodies, Monoclonal - chemistry
Antibodies, Monoclonal, Humanized
Bevacizumab
Conjugation
Diagnostic Imaging - methods
Drug Delivery Systems - methods
Encapsulation
Enzyme-Linked Immunosorbent Assay
Exudation
Growth factors
Humans
Imaging
Logistic Models
Macular Degeneration - drug therapy
Microbubbles
Microorganisms
Microscopy, Electron, Scanning
Therapy
Ultrasound
Vascular Endothelial Growth Factor A - antagonists & inhibitors
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Summary:We synthesize multifunctional microbubbles (MBs) for targeted delivery of antivascular endothelial growth factor (antiVEGF) therapy with multimodal imaging guidance. Poly-lactic-co-glycolic acid (PLGA) MBs encapsulating Texas Red dye are fabricated by a modified double-emulsion process. Simultaneous ultrasound and fluorescence imaging are achieved using Texas Red encapsulated MBs. The MBs are conjugated with Avastin, an antiVEGF antibody for treating neovascular age-related macular degeneration (AMD). The conjugation efficiency is characterized by enzyme-linked immunosorbent assay (ELISA). The efficiency for targeted binding of Avastin-conjugated MBs is characterized by microscopic imaging. Our work demonstrates the technical potential of using multifunctional MBs for targeted delivery of antiVEGF therapy in the treatment of exudative AMD.
ISSN:1083-3668
1560-2281
DOI:10.1117/1.3457669