Illumination and fluorescence collection volumes for fiber optic probes in tissue

Optical fibers can deliver light to, and collect it from, regions deep in tissue. However, reported illumination and fluorescence collection volumes adjacent to the fiber tip have been inconsistent, and systematic data on this topic are not available. Illumination and fluorescence collection profile...

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Bibliographic Details
Published in:Journal of Biomedical Optics 2007-05, Vol.12 (3), p.034033-0340312
Main Authors: Tai, Dean C.S, Hooks, Darren A, Harvey, John D, Smaill, Bruce H, Soeller, Christian
Format: Article
Language:English
Subjects:
Animals
Collection
collection volume
Equipment Design
Equipment Failure Analysis
Fiber Optic Technology - instrumentation
Fiber optics
Fibers
Fluid flow
Fluorescence
fluorescence imaging
Heart - physiology
Illumination
illumination volume
imaging system
In Vitro Techniques
intramural measurement
Lighting - instrumentation
Mathematical models
Optical Fibers
optical probe
Rats
Rats, Inbred WKY
Reproducibility of Results
Sensitivity and Specificity
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Summary:Optical fibers can deliver light to, and collect it from, regions deep in tissue. However, reported illumination and fluorescence collection volumes adjacent to the fiber tip have been inconsistent, and systematic data on this topic are not available. Illumination and fluorescence collection profiles were characterized with high spatial resolution for different optical fibers in tissue and various fluids using two-photon flash photolysis and excitation. We confirm that illumination and fluorescence collection volumes for optical fibers are near identical. Collection volume is determined by the core dimensions and numerical aperture (NA) of the fiber and the scattering properties of the medium. For a multimode optical fiber with core diam and , 80 of the total fluorescence is collected from a depth of in tissue and in nonscattering fluid. A semiempirical mathematical description of photon flux adjacent to the fiber tip was also developed and validated. This was used to quantify the extent of temporal blurring associated with propagation of a wavefront of altered fluorescence emission across the region addressed by fiber optic probes. We provide information that will facilitate the design of optical probes for tissue imaging or therapeutic applications.
ISSN:1083-3668
1560-2281
DOI:10.1117/1.2750288