Multicontrast microscopy technique to dynamically fingerprint live-cell focal contacts during exposure and replacement of a cytotoxic medium

Multicontrast microscopy techniques were used to comprehensively and dynamically map the cellular contact area adhering to a substrate. The natural fringe patterns observed with interference reflection contrast microscopy were used to map the dynamic fingerprint of a porcine pulmonary artery endothe...

Full description

Saved in:
Bibliographic Details
Published in:Journal of Biomedical Optics 2008-09, Vol.13 (5), p.054069-054065
Main Authors: Choi, Chang K, Margraves, Charles H, English, Anthony E, Kihm, Kenneth D
Format: Article
Language:English
Subjects:
Animals
Biocompatibility
Cell Adhesion - drug effects
Cell Adhesion - physiology
Cells, Cultured
Cellular
Cytochalasin D - administration & dosage
cytotoxicity
Cytotoxins - administration & dosage
dynamic cellular fingerprinting
endothelial cells
Epithelial Cells - cytology
Epithelial Cells - drug effects
Epithelial Cells - physiology
Fingerprints
focal contacts
Image Enhancement - methods
Interference
interference reflection contrast microscopy
Microscopy
Microscopy, Phase-Contrast - methods
Reagents
Reflection
Swine
Toxicology
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Multicontrast microscopy techniques were used to comprehensively and dynamically map the cellular contact area adhering to a substrate. The natural fringe patterns observed with interference reflection contrast microscopy were used to map the dynamic fingerprint of a porcine pulmonary artery endothelial cell's ventral surface and to examine the focal and/or close contacts to the substrate when exposed to a toxic agent Cytochalasin D. In addition, differential interference contrast microscopy sequentially imaged the overall cellular morphological responses to the agent. It was observed that focal contacts, which are tightly attached to the substrate, are strongly resistant to even high doses of the cytotoxic agent and that they also form the basis of cellular recovery after replacement of the cytotoxic medium with fresh medium.
ISSN:1083-3668
1560-2281
DOI:10.1117/1.2993143