Delivery of fluorescent probes using iron oxide particles as carriers enables in-vivo labeling of migrating neural precursors for magnetic resonance imaging and optical imaging

Iron oxide particles are becoming an important contrast agent for magnetic resonance imaging (MRI) cell tracking studies. Simultaneous delivery of fluorescence indicators with the particles to individual cells offers the possibility of correlating optical images and MRI. In this work, it is demonstr...

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Bibliographic Details
Published in:Journal of biomedical optics 2007-09, Vol.12 (5), p.051504-051504
Main Authors: Sumner, James P, Conroy, Richard, Shapiro, Erik M, Moreland, John, Koretsky, Alan P
Format: Article
Language:English
Subjects:
Animals
Biomedical materials
Brain - cytology
Carriers
Cell Movement - physiology
Ferric Compounds
Fluorescent Dyes
Image Enhancement - methods
Imaging
Iron oxides
Magnetic resonance imaging
Magnetic Resonance Imaging - methods
Microscopy, Fluorescence - methods
Molecular Probe Techniques
Neurons - cytology
Neurons - physiology
Rats
Rats, Sprague-Dawley
Staining and Labeling - methods
Surgical implants
Tracking
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Summary:Iron oxide particles are becoming an important contrast agent for magnetic resonance imaging (MRI) cell tracking studies. Simultaneous delivery of fluorescence indicators with the particles to individual cells offers the possibility of correlating optical images and MRI. In this work, it is demonstrated that micron-sized iron oxide particles (MPIOs) can be used as a carrier to deliver fluorescent probes to cells in culture as well as to migrating neural progenitors in vivo. Migrating progenitors were tracked with MRI and easily identified by histology because of the fluorescent probe. These data suggest that using MPIOs to deliver fluorescent probes should make it possible to combine MRI and optical imaging for in vivo cell tracking.
ISSN:1083-3668
DOI:10.1117/1.2800294