Automated three-dimensional tracking of living cells by digital holographic microscopy

Digital holographic microscopy (DHM) enables a quantitative multifocus phase contrast imaging that has been found suitable for technical inspection and quantitative live cell imaging. The combination of DHM with fast and robust autofocus algorithms enables subsequent automated focus realignment by n...

Full description

Saved in:
Bibliographic Details
Published in:Journal of Biomedical Optics 2009-01, Vol.14 (1), p.014018-014017
Main Authors: Langehanenberg, Patrik, Ivanova, Lyubomira, Bernhardt, Ingolf, Ketelhut, Steffi, Vollmer, Angelika, Dirksen, Dieter, Georgiev, Georgi, von Bally, Gert, Kemper, Bjo¨rn
Format: Article
Language:English
Subjects:
Algorithms
Artificial Intelligence
Automated
automated three-dimensional cell tracking
Cell Movement - physiology
Cells, Cultured
Digital
digital holographic microscopy
Erythrocytes - cytology
Erythrocytes - physiology
Holography - methods
Humans
Image contrast
Image Enhancement - methods
Image Interpretation, Computer-Assisted - methods
Imaging
Imaging, Three-Dimensional - methods
live cell imaging
Microscopy
Microscopy - methods
Pattern Recognition, Automated - methods
Phase contrast
Reproducibility of Results
Sensitivity and Specificity
Signal Processing, Computer-Assisted
Tracking
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Digital holographic microscopy (DHM) enables a quantitative multifocus phase contrast imaging that has been found suitable for technical inspection and quantitative live cell imaging. The combination of DHM with fast and robust autofocus algorithms enables subsequent automated focus realignment by numerical propagation of the digital holographically reconstructed object wave. In combination with a calibrated optical imaging system, the obtained propagation data quantify axial displacements of the investigated sample. The evaluation of quantitative DHM phase contrast images also enables an effective determination of lateral cell displacements. Thus, 3-D displacement data are provided. Results from investigations on sedimenting red blood cells and HT-1080 fibrosarcoma cells in a collagen tissue model demonstrate that DHM enables marker-free automated quantitative dynamic 3-D cell tracking without mechanical focus adjustment.
ISSN:1083-3668
1560-2281
DOI:10.1117/1.3080133