Serum protein profile study of normal and cervical cancer subjects by high performance liquid chromatography with laser-induced fluorescence

High performance liquid chromatography with high sensitivity laser-induced fluorescence detection is used to study the protein profiles of serum samples from healthy volunteers and cervical cancer subjects. The protein profiles are subjected to principal component analysis (PCA). PCA shows that the...

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Bibliographic Details
Published in:Journal of Biomedical Optics 2008-09, Vol.13 (5), p.054062-0540610
Main Authors: Sujatha, Rai, Lavanya, Kumar, Pratap, Mahato, Krishna K, Kartha, Vasudevan B, Santhosh, Chidangil
Format: Article
Language:English
Subjects:
Adult
Aged
Biomarkers, Tumor - blood
Blood Chemical Analysis - methods
Calibration
Cancer
cervical cancer
Chromatography, High Pressure Liquid - methods
Female
Fluorescence
High performance liquid chromatography
high performance liquid chromatography-laser induced fluorescence
Humans
Lasers
Mahalanobis distance
Middle Aged
Neoplasm Proteins - blood
principal component analysis
protein profile
Proteins
Reproducibility of Results
Sensitivity and Specificity
serum
Serum proteins
Serums
Spectrometry, Fluorescence - methods
Uterine Cervical Neoplasms - blood
Uterine Cervical Neoplasms - diagnosis
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Summary:High performance liquid chromatography with high sensitivity laser-induced fluorescence detection is used to study the protein profiles of serum samples from healthy volunteers and cervical cancer subjects. The protein profiles are subjected to principal component analysis (PCA). PCA shows that the large number of chromatograms of a given class of serum samples-say normal/malignant-can be expressed in terms of a small number of factors (principal components). Three parameters-scores of the factors, squared residuals, and Mahalanobis distance-are derived from PCA. The parameters are observed to have a narrow range for protein profiles of standard calibration sets formed from groups of clinically confirmed normal/malignant classes. Limit tests using match/no match of the parameters of any test sample with parameters derived for the standard calibration sets give very good discrimination between malignant and normal samples with high sensitivity and specificity .
ISSN:1083-3668
1560-2281
DOI:10.1117/1.2992166