Multimodal optical microscope for detecting viability of mouse embryos in vitro

We present a multimodal optical microscope that incorporates six imaging modalities on one common platform. The imaging modalities include three staring modes, optical quadrature microscopy (OQM), differential interference contrast (DIC) microscopy, and epi-fluorescence microscopy, and three scannin...

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Bibliographic Details
Published in:Journal of biomedical optics 2007-07, Vol.12 (4), p.044006-044006
Main Authors: Warger, 2nd, William C, Laevsky, Gary S, Townsend, Daniel J, Rajadhyaksha, Milind, DiMarzio, Charles A
Format: Article
Language:English
Subjects:
Animals
Confocal
Embryo, Mammalian - cytology
Embryo, Mammalian - physiology
Embryos
Equipment Design
Equipment Failure Analysis
Fetal Viability - physiology
Illumination
Image Enhancement - instrumentation
Image Enhancement - methods
Image Interpretation, Computer-Assisted - instrumentation
Image Interpretation, Computer-Assisted - methods
Imaging
Mice
Microscopy
Microscopy, Confocal - instrumentation
Microscopy, Confocal - methods
Microscopy, Fluorescence - instrumentation
Microscopy, Fluorescence - methods
Microscopy, Polarization - instrumentation
Microscopy, Polarization - methods
Optical microscopes
Scanning
Systems Integration
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Summary:We present a multimodal optical microscope that incorporates six imaging modalities on one common platform. The imaging modalities include three staring modes, optical quadrature microscopy (OQM), differential interference contrast (DIC) microscopy, and epi-fluorescence microscopy, and three scanning modes, confocal reflectance microscopy (CRM), confocal fluorescence microscopy (CFM), and two-photon microscopy (2PM). OQM reconstructs the amplitude and phase of an optically transparent specimen within a modified Mach-Zehnder configuration. DIC microscopy images the phase gradient along a specified direction of an optically transparent specimen. CRM detects index of refraction changes that modulate backscatter. Epi-fluorescence microscopy, CFM, and 2PM detect endogenous and exogenous fluorophores within a specimen. The scanning modes are inherently capable of producing three-dimensional (3-D) images due to optical sectioning and localized probing. Illumination and imaging are performed coaxially with minimal changes of optical components between modes. Multimodal images of embryos are shown to demonstrate the microscope's imaging capabilities.
ISSN:1083-3668
DOI:10.1117/1.2753744