NAT2 slow acetylation haplotypes are associated with the increased risk of betel quid–related oral and pharyngeal squamous cell carcinoma

• Published in: Oral surgery, oral medicine, oral pathology, oral radiology and endodontics oral medicine, oral pathology, oral radiology and endodontics, 2011-10, Vol.112 (4), p.484-492
• Main Authors: Hou, Yu-Yi, MD, Ou, Hui-Ling, MS, Chu, Sau-Tung, MD, Wu, Pi-Chuang, MS, Lu, Pei-Jung, PhD, Chi, Chao-Chuan, MS, MD, Leung, Kam-Wing, MD, Lee, Chien-Yiing, MD, Wu, Pi-Hsiung, MD, Hsiao, Michael, PhD, Ger, Luo-Ping, MPH
• Format: Article
• Language: English
• Subjects: Acetylation; Adenine; Adult; Age Factors; Alcohol Drinking; Areca - adverse effects; Arylamine N-Acetyltransferase - genetics; Biological and medical sciences; Carcinoma, Squamous Cell - genetics; Case-Control Studies; Cytosine; Dentistry; Gene Frequency - genetics; Genetic Markers - genetics; Genotype; Guanine; Haplotypes - genetics; Humans; Linkage Disequilibrium - genetics; Male; Medical sciences; Middle Aged; Mouth Neoplasms - genetics; Otorhinolaryngology. Stomatology; Pharyngeal Neoplasms - genetics; Polymorphism, Restriction Fragment Length - genetics; Polymorphism, Single Nucleotide - genetics; Risk Factors; Smoking; Surgery; Thymine; Tumors; Upper respiratory tract, upper alimentary tract, paranasal sinuses, salivary glands: diseases, semeiology
• ISSN: 1079-2104; 1528-395X
• DOI: 10.1016/j.tripleo.2011.03.036

Background NAT2, the most important phase II metabolic enzyme for betel quid (BQ), might modify the risk of BQ-related oral and pharyngeal squamous cell carcinoma (OPSCC) in Taiwan. Study design PCR-RFLP and TaqMan assay were conducted for genotyping of NAT2 in 172 OPSCC cases and 170 healthy controls who habitually chewed BQ. Results The genotypic and allelic type of T341C and C481T in NAT2 are associated with the risk of OPSCC. There were linear trends between increased risk of OPSCC and slowness of NAT2 acetylation haplotypes ( P = .017), especially for young subjects ( P < .001), light BQ chewers ( P = .005), light smokers ( P = .023), and alcohol drinkers ( P = .001). The interactions on risk of OPSCC were found for NAT2 acetylation haplotypes with status of age, BQ chewing, and alcohol drinking. Conclusions The NAT2 acetylation haplotypes might be genetic markers for risk of BQ-related OPSCC.