ZmCBF3 overexpression improves tolerance to abiotic stress in transgenic rice (Oryza sativa) without yield penalty

Plant productivity is greatly affected by environmental stresses such as drought, salt, and freezing. We previously described a C-repeat binding transcription factor from maize (ZmCBF3) that was upregulated by both abscisic acid and low-temperature and actively expressed during embryogenesis. To und...

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Bibliographic Details
Published in:Plant cell reports 2011-10, Vol.30 (10), p.1949-1957
Main Authors: Xu, Miaoyun, Li, Luhui, Fan, Yunliu, Wan, Jianmin, Wang, Lei
Format: Article
Language:English
Subjects:
Abscisic acid
Abscisic Acid - pharmacology
Biological and medical sciences
Biomedical and Life Sciences
Biotechnology
Cell Biology
Cold Temperature
Drought
Droughts
Embryogenesis
Embryonic growth stage
Environmental stress
Freezing
Fundamental and applied biological sciences. Psychology
Gene Expression Regulation, Plant
Grain
Growth conditions
Growth rate
Life Sciences
Low temperature
Malondialdehyde
Malondialdehyde - analysis
Original Paper
Oryza - genetics
Oryza - growth & development
Oryza sativa
Plant Biochemistry
Plant Proteins - genetics
Plant Proteins - metabolism
Plant Sciences
Plants, Genetically Modified - genetics
Plants, Genetically Modified - growth & development
Promoters
Rice
Salinity
Salts
Stress, Physiological
Survival
Transcription
Transcription factors
Transcription Factors - genetics
Transcription Factors - metabolism
Transformation, Genetic
Transgenic plants
Ubiquitin
Zea mays
Zea mays - genetics
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Summary:Plant productivity is greatly affected by environmental stresses such as drought, salt, and freezing. We previously described a C-repeat binding transcription factor from maize (ZmCBF3) that was upregulated by both abscisic acid and low-temperature and actively expressed during embryogenesis. To understand the stress response in rice, transgenic ZmCBF3 rice with ubiquitin promoter was developed. T3 generation was planted and analyzed. The results showed that overexpression of ZmCBF3 in rice did not cause growth retardation under normal growth conditions with improved tolerance to drought, high-salt, and low-temperature stresses. Moreover, the transgenic rice grain yield was similar to wild type plants under normal conditions. The transgenic plants showed enhanced survival rate and reduced malondialdehyde content and relative conductivity under drought, salt, and low-temperature stresses. ZmCBF3 overexpression in transgenic rice increased the transcript levels of stress-induced genes and enhanced the tolerance to drought, salt, and low-temperature stresses.
ISSN:0721-7714
1432-203X
DOI:10.1007/s00299-011-1103-1