Fast CEC‐MS using poly(dimethylsiloxane) microinjector, short packed column, and low‐sheath‐flow interface

• Published in: Journal of separation science 2011-09, Vol.34 (18), p.2538-2543
• Main Authors: Chen, Chao‐Jung, Wang, Che‐Wei, Her, Guor‐Rong
• Format: Article
• Language: English
• Subjects: Analytical chemistry; Biological and medical sciences; Capillarity; Capillary Electrochromatography; CEC-MS; Chemical control; Chemistry; Chromatographic methods and physical methods associated with chromatography; Column packings; Devices; Dimethylpolysiloxanes - chemistry; Elution; Exact sciences and technology; Fundamental and applied biological sciences. Psychology; Liquids; Low-sheath-flow interface; Mass Spectrometry; Microinjections - instrumentation; Microinjections - methods; On-line systems; Online; Online pre-concentration; Other chromatographic methods; Packed CEC; Packed columns; Parasitic plants. Weeds; PDMS; Phytopathology. Animal pests. Plant and forest protection; separation; silica; triazines; Triazines - analysis; Weeds
• ISSN: 1615-9306; 1615-9314; 1615-9314
• DOI: 10.1002/jssc.201100281

A fast CEC‐MS approach based on a microinjector and a short CEC column was developed. Poly(dimethylsiloxane) was used as the substrate for microinjector fabrication. A short capillary column (∼5 cm) packed with 5 μm octadecyl silica particles was inserted into the microinjector. The microinjector CEC device was interfaced to ESI‐MS using a low‐flow sheath liquid interface. The device delivers the advantages of sample introduction, pre‐concentration, elution, and fast analysis as in chip‐CEC yet avoids the difficulty of packing stationary material into the chip. The online pre‐concentration and CEC‐MS analysis capabilities of this device were demonstrated by analysis of a six‐triazine mixture. A signal enhancement of 20–99‐fold was achieved with a sample loading time of 180 s.