Observation of In Vivo Cytochrome-Based Electron-Transport Dynamics Using Time-Resolved Evanescent Wave Electroabsorption Spectroscopy

Morning light: In vivo photodissociation of CO from bacterial c‐type cytochromes yields a redox‐active Fe2+ form, which can be oxidized at an electrode surface to the Fe3+ form. Reduction by electrons from the metabolic pathway regenerates the Fe2+ form (see picture). Spectroscopic monitoring of thi...

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Bibliographic Details
Published in:Angewandte Chemie International Edition 2011-09, Vol.50 (39), p.9137-9140
Main Authors: Shibanuma, Toshihiko, Nakamura, Ryuhei, Hirakawa, Yuichiro, Hashimoto, Kazuhito, Ishii, Kazuyuki
Format: Article
Language:English
Subjects:
Carbon Monoxide - chemistry
cytochromes
Cytochromes c - chemistry
Electrodes
electron transfer
Electron Transport
Ferric Compounds - chemistry
Ferrous Compounds - chemistry
in vivo studies
Oxidation-Reduction
Photolysis
Shewanella - metabolism
Spectrophotometry, Ultraviolet
Time Factors
time-resolved spectroscopy
UV/Vis spectroscopy
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Summary:Morning light: In vivo photodissociation of CO from bacterial c‐type cytochromes yields a redox‐active Fe2+ form, which can be oxidized at an electrode surface to the Fe3+ form. Reduction by electrons from the metabolic pathway regenerates the Fe2+ form (see picture). Spectroscopic monitoring of this process yields information on the in vivo respiratory electron‐transport dynamics.
ISSN:1433-7851
1521-3773
DOI:10.1002/anie.201101810