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Application of flow cytometry for the identification of Staphylococcus epidermidis by peptide nucleic acid fluorescence in situ hybridization (PNA FISH) in blood samples
Staphylococcus epidermidis is considered to be one of the most common causes of nosocomial bloodstream infections, particularly in immune-compromised individuals. Here, we report the development and application of a novel peptide nucleic acid probe for the specific detection of S. epidermidis by flu...
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| Published in: | Antonie van Leeuwenhoek 2011-10, Vol.100 (3), p.463-470 |
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| Main Authors: | , , , , , , , |
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| cites | cdi_FETCH-LOGICAL-c468t-1a95e231fdfd079f74f1dd7dcffbc459c52aff9c69114e6f43bd1eb3b663aa853 |
| container_end_page | 470 |
| container_issue | 3 |
| container_start_page | 463 |
| container_title | Antonie van Leeuwenhoek |
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| creator | Azevedo, N. F. Jardim, T. Almeida, C. Cerqueira, L. Almeida, A. J. Rodrigues, F. Keevil, C. W. Vieira, M. J. |
| description | Staphylococcus epidermidis
is considered to be one of the most common causes of nosocomial bloodstream infections, particularly in immune-compromised individuals. Here, we report the development and application of a novel peptide nucleic acid probe for the specific detection of
S. epidermidis
by fluorescence in situ hybridization. The theoretical estimates of probe matching specificity and sensitivity were 89 and 87%, respectively. More importantly, the probe was shown not to hybridize with closely related species such as
Staphylococcus aureus
. The method was subsequently successfully adapted for the detection of
S. epidermidis
in mixed-species blood cultures both by microscopy and flow cytometry. |
| doi_str_mv | 10.1007/s10482-011-9595-9 |
| format | article |
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is considered to be one of the most common causes of nosocomial bloodstream infections, particularly in immune-compromised individuals. Here, we report the development and application of a novel peptide nucleic acid probe for the specific detection of
S. epidermidis
by fluorescence in situ hybridization. The theoretical estimates of probe matching specificity and sensitivity were 89 and 87%, respectively. More importantly, the probe was shown not to hybridize with closely related species such as
Staphylococcus aureus
. The method was subsequently successfully adapted for the detection of
S. epidermidis
in mixed-species blood cultures both by microscopy and flow cytometry.</description><identifier>ISSN: 0003-6072</identifier><identifier>EISSN: 1572-9699</identifier><identifier>DOI: 10.1007/s10482-011-9595-9</identifier><identifier>PMID: 21638111</identifier><identifier>CODEN: ANLEDR</identifier><language>eng</language><publisher>Dordrecht: Springer Netherlands</publisher><subject>Bacteria ; Bacteriological methods and techniques used in bacteriology ; Bacteriology ; Biological and medical sciences ; Biomedical and Life Sciences ; Blood ; Blood - microbiology ; Cross Infection - diagnosis ; Cross Infection - microbiology ; Flow cytometry ; Flow Cytometry - methods ; Fluorescence ; Fluorescence in situ hybridization ; Fundamental and applied biological sciences. Psychology ; Humans ; Hybridization ; In Situ Hybridization, Fluorescence - methods ; Life Sciences ; Medical Microbiology ; Microbiology ; Nosocomial infection ; Nosocomial infections ; Nucleic acids ; Peptide Nucleic Acids - genetics ; Plant Sciences ; Short Communication ; Soil Science & Conservation ; Staphylococcal Infections - diagnosis ; Staphylococcal Infections - microbiology ; Staphylococcus epidermidis - genetics ; Staphylococcus epidermidis - isolation & purification</subject><ispartof>Antonie van Leeuwenhoek, 2011-10, Vol.100 (3), p.463-470</ispartof><rights>Springer Science+Business Media B.V. 2011</rights><rights>2015 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c468t-1a95e231fdfd079f74f1dd7dcffbc459c52aff9c69114e6f43bd1eb3b663aa853</citedby><cites>FETCH-LOGICAL-c468t-1a95e231fdfd079f74f1dd7dcffbc459c52aff9c69114e6f43bd1eb3b663aa853</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27923,27924</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=24523988$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21638111$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Azevedo, N. F.</creatorcontrib><creatorcontrib>Jardim, T.</creatorcontrib><creatorcontrib>Almeida, C.</creatorcontrib><creatorcontrib>Cerqueira, L.</creatorcontrib><creatorcontrib>Almeida, A. J.</creatorcontrib><creatorcontrib>Rodrigues, F.</creatorcontrib><creatorcontrib>Keevil, C. W.</creatorcontrib><creatorcontrib>Vieira, M. J.</creatorcontrib><title>Application of flow cytometry for the identification of Staphylococcus epidermidis by peptide nucleic acid fluorescence in situ hybridization (PNA FISH) in blood samples</title><title>Antonie van Leeuwenhoek</title><addtitle>Antonie van Leeuwenhoek</addtitle><addtitle>Antonie Van Leeuwenhoek</addtitle><description>Staphylococcus epidermidis
is considered to be one of the most common causes of nosocomial bloodstream infections, particularly in immune-compromised individuals. Here, we report the development and application of a novel peptide nucleic acid probe for the specific detection of
S. epidermidis
by fluorescence in situ hybridization. The theoretical estimates of probe matching specificity and sensitivity were 89 and 87%, respectively. More importantly, the probe was shown not to hybridize with closely related species such as
Staphylococcus aureus
. The method was subsequently successfully adapted for the detection of
S. epidermidis
in mixed-species blood cultures both by microscopy and flow cytometry.</description><subject>Bacteria</subject><subject>Bacteriological methods and techniques used in bacteriology</subject><subject>Bacteriology</subject><subject>Biological and medical sciences</subject><subject>Biomedical and Life Sciences</subject><subject>Blood</subject><subject>Blood - microbiology</subject><subject>Cross Infection - diagnosis</subject><subject>Cross Infection - microbiology</subject><subject>Flow cytometry</subject><subject>Flow Cytometry - methods</subject><subject>Fluorescence</subject><subject>Fluorescence in situ hybridization</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Humans</subject><subject>Hybridization</subject><subject>In Situ Hybridization, Fluorescence - methods</subject><subject>Life Sciences</subject><subject>Medical Microbiology</subject><subject>Microbiology</subject><subject>Nosocomial infection</subject><subject>Nosocomial infections</subject><subject>Nucleic acids</subject><subject>Peptide Nucleic Acids - genetics</subject><subject>Plant Sciences</subject><subject>Short Communication</subject><subject>Soil Science & Conservation</subject><subject>Staphylococcal Infections - diagnosis</subject><subject>Staphylococcal Infections - microbiology</subject><subject>Staphylococcus epidermidis - genetics</subject><subject>Staphylococcus epidermidis - isolation & purification</subject><issn>0003-6072</issn><issn>1572-9699</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><recordid>eNp10d1qFDEYBuAgit1WL8ATCYKoB6P55i_J4VKsLRQVqschkx83JTMZkwwy3pF32Sy7WhE8CkmevPngRegZkLdACH2XgLSsrghAxTveVfwB2kBH64r3nD9EG0JIU_WE1ifoNKXbsuU9o4_RSQ19wwBgg35t59k7JbMLEw4WWx9-YLXmMJocV2xDxHlnsNNmys7-BW-ynHerDyootSRs5kLi6LRLeFjxbOZcDvC0KG-cwlI5XbKXEE1SZlIlccLJ5QXv1iGWVz8Pwa8_f9zii6ubyzd7MPgQNE5ynL1JT9AjK30yT4_rGfp68f7L-WV1_enD1fn2ulJtz3IFknembsBqqwnllrYWtKZaWTuotuOqq6W1XPUcoDW9bZtBgxmaoe8bKVnXnKFXh9w5hu-LSVmMrszsvZxMWJJgnPSUcsqLfPGPvA1LnMpwgjHeFgJQEByQiiGlaKyYoxtlXAUQsW9RHFoUpUWxb1Hsg58fg5dhNPrPi9-1FfDyCGRS0tsoJ-XSvWu7uuGMFVcfXCpX0zcT7yf8_-93H3G4fg</recordid><startdate>20111001</startdate><enddate>20111001</enddate><creator>Azevedo, N. 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Psychology</topic><topic>Humans</topic><topic>Hybridization</topic><topic>In Situ Hybridization, Fluorescence - methods</topic><topic>Life Sciences</topic><topic>Medical Microbiology</topic><topic>Microbiology</topic><topic>Nosocomial infection</topic><topic>Nosocomial infections</topic><topic>Nucleic acids</topic><topic>Peptide Nucleic Acids - genetics</topic><topic>Plant Sciences</topic><topic>Short Communication</topic><topic>Soil Science & Conservation</topic><topic>Staphylococcal Infections - diagnosis</topic><topic>Staphylococcal Infections - microbiology</topic><topic>Staphylococcus epidermidis - genetics</topic><topic>Staphylococcus epidermidis - isolation & purification</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Azevedo, N. F.</creatorcontrib><creatorcontrib>Jardim, T.</creatorcontrib><creatorcontrib>Almeida, C.</creatorcontrib><creatorcontrib>Cerqueira, L.</creatorcontrib><creatorcontrib>Almeida, A. 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is considered to be one of the most common causes of nosocomial bloodstream infections, particularly in immune-compromised individuals. Here, we report the development and application of a novel peptide nucleic acid probe for the specific detection of
S. epidermidis
by fluorescence in situ hybridization. The theoretical estimates of probe matching specificity and sensitivity were 89 and 87%, respectively. More importantly, the probe was shown not to hybridize with closely related species such as
Staphylococcus aureus
. The method was subsequently successfully adapted for the detection of
S. epidermidis
in mixed-species blood cultures both by microscopy and flow cytometry.</abstract><cop>Dordrecht</cop><pub>Springer Netherlands</pub><pmid>21638111</pmid><doi>10.1007/s10482-011-9595-9</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Antonie van Leeuwenhoek, 2011-10, Vol.100 (3), p.463-470 |
| issn | 0003-6072 1572-9699 |
| language | eng |
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| source | Springer Nature |
| subjects | Bacteria Bacteriological methods and techniques used in bacteriology Bacteriology Biological and medical sciences Biomedical and Life Sciences Blood Blood - microbiology Cross Infection - diagnosis Cross Infection - microbiology Flow cytometry Flow Cytometry - methods Fluorescence Fluorescence in situ hybridization Fundamental and applied biological sciences. Psychology Humans Hybridization In Situ Hybridization, Fluorescence - methods Life Sciences Medical Microbiology Microbiology Nosocomial infection Nosocomial infections Nucleic acids Peptide Nucleic Acids - genetics Plant Sciences Short Communication Soil Science & Conservation Staphylococcal Infections - diagnosis Staphylococcal Infections - microbiology Staphylococcus epidermidis - genetics Staphylococcus epidermidis - isolation & purification |
| title | Application of flow cytometry for the identification of Staphylococcus epidermidis by peptide nucleic acid fluorescence in situ hybridization (PNA FISH) in blood samples |
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