Reference Values of Fetal Erythrocytes in Maternal Blood During Pregnancy Established Using Flow Cytometry

The aim of our study was to assess the fetal RBC count in maternal blood during uncomplicated pregnancies from 26 weeks onward. We used a flow cytometric method specifically designed for use in a routine hematology analyzer. Pregnant women were recruited through midwives. The participating laborator...

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Bibliographic Details
Published in:American journal of clinical pathology 2011-10, Vol.136 (4), p.631-636
Main Authors: DE WIT, Harry, NABBE, Karin C. A. M, KOOREN, Jurgen A, ADRIAANSEN, HenkJ, ROELANDSE-KOOP, Elianne A, SCHUITEMAKER, Joost H. N, HOFFMANN, Johannes J. M. L
Format: Article
Language:English
Subjects:
Biological and medical sciences
Cell Separation
Erythrocyte Count - methods
Erythrocytes - cytology
Female
Fetomaternal Transfusion - blood
Fetus
Flow Cytometry - methods
Humans
Investigative techniques, diagnostic techniques (general aspects)
Medical sciences
Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques
Pregnancy - blood
Reference Values
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Summary:The aim of our study was to assess the fetal RBC count in maternal blood during uncomplicated pregnancies from 26 weeks onward. We used a flow cytometric method specifically designed for use in a routine hematology analyzer. Pregnant women were recruited through midwives. The participating laboratories used the FMH QuikQuant method (Trillium Diagnostics, Brewer, ME) in a CELL-DYN Sapphire hematology analyzer (Abbott Diagnostics, Santa Clara, CA). The method is based on a monoclonal antibody to hemoglobin F. Flow cytometric data were analyzed by 2 independent observers. The 95th percentile reference range was estimated according to Clinical and Laboratory Standards Institute guidelines. A total of 236 samples were statistically analyzed. Gestational ages ranged from 21.6 to 41 weeks (mean, 32.0 weeks), and the fetal RBC count in maternal blood ranged from 0.00% to 0.50% (median, 0.025%). The fetal RBC count in maternal blood shows no correlation with gestational age. The established reference range during normal pregnancy is less than 0.125%.
ISSN:0002-9173
1943-7722
DOI:10.1309/ajcphl3vxy0vmlxl